Hydroxyurea reversal of inhibition and use as a cell-synchronizing agent.
Hydroxyurea reversal of inhibition and use as a cell-synchronizing agent.
复制标题
羟基脲逆转抑制并用作细胞同步剂。
DOI：
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发表时间：
1967
影响因子：
4.8
通讯作者：
J. Lindsay
中科院分区：
文献类型：
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作者：
R. Adams;J. Lindsay
摘要羟基脲通过抑制核糖核苷酸还原酶抑制小鼠成纤维细胞脱氧核糖核酸的合成。没有发现其他作用部位，抑制作用可以通过去除药物或加入脱氧核苷来逆转。虽然嘧啶脱氧核糖核苷容易被细胞磷酸化，但相应的嘌呤化合物大部分转化为核糖核苷酸。在羟基脲中孵育18小时后，洗去抑制剂的L细胞显示持续约8小时的DNA合成爆发，涉及70%的细胞。
Abstract Hydroxyurea inhibits deoxyribonucleic acid synthesis in mouse fibroblast (L) cells by inhibiting ribonucleotide reductase. No other site of action could be found. Inhibition can be reversed either by removal of the drug or by addition of deoxyribonucleosides. Although pyrimidine deoxyribonucleosides are readily phosphorylated by the cells, the corresponding purine compounds are largely converted to ribonucleotides. Following incubation for 18 hours in hydroxyurea, L cells washed free of inhibitor showed a burst of DNA synthesis lasting about 8 hours and involving 70% of the cells.