Sclerenchyma cell thickening through enhanced lignification induced by OsMYB30 prevents fungal penetration of rice leaves
Sclerenchyma cell thickening through enhanced lignification induced by OsMYB30 prevents fungal penetration of rice leaves
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OsMYB30 诱导的木质化增强，厚壁组织细胞增厚，防止真菌渗透稻叶
DOI：
10.1111/nph.16505
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发表时间：
2020-03-31
期刊：
影响因子：
9.4
通讯作者：
Chen, Xuewei
中科院分区：
文献类型：
--
作者：
Li, Weitao;Wang, Kang;Chen, Xuewei
在作物育种方案中，广谱抗性是高度优先的。在此之前，我们已经报道了从水稻地谷中鉴定出广谱抗性-地谷1（bsr-d1）等位基因。bsr-d1等位基因阻止稻瘟病菌感染激活Bsr-d1表达和过氧化物酶降解H2 O2，导致对稻瘟病菌的抗性。米。然而，它仍然是未知的防御途径以外的H2 O2爆发和过氧化物酶是否有助于bsr-d1介导的immunity.稻瘟病抗性测定叶片喷雾和打孔接种。通过酵母单杂交和电泳迁移率变动分析鉴定OsMYB 30的目的基因。木质素含量通过间苯三酚-盐酸染色法、乙酰溴法和硫代酸解法测定。OsMYB 30在M.当Bsr-d1被敲除或下调时，如在bsr-d1植物中感染时发生的。我们进一步发现OsMYB 30结合并激活4-香豆酸：辅酶A连接酶基因（Os 4CL 3和Os 4CL 5）的启动子，导致木质素亚基G和S的积累。这一作用导致表皮附近厚壁组织细胞明显增厚，抑制了M.我们的研究揭示了bsr-d1介导的抗性和穿透依赖性免疫所需的新成分，并推进了我们对广谱抗病性的理解。
Broad-spectrum resistance is highly preferred in crop breeding programmes. Previously, we have reported the identification of the broad-spectrum resistance-Digu 1 (bsr-d1) allele from rice Digu. The bsr-d1 allele prevents activation of Bsr-d1 expression by Magnaporthe oryzae infection and degradation of H2O2 by peroxidases, leading to resistance to M. oryzae. However, it remains unknown whether defence pathways other than H2O2 burst and peroxidases contribute to the bsr-d1-mediated immunity.Blast resistance was determined in rice leaves by spray and punch inoculations. Target genes of OsMYB30 were identified by one-hybrid assays in yeast and electrophoretic mobility shift assay. Lignin content was measured by phloroglucinol-HCl staining, and acetyl bromide and thioacidolysis methods.Here, we report the involvement of the OsMYB30 gene in bsr-d1-mediated blast resistance. Expression of OsMYB30 was induced during M. oryzae infection or when Bsr-d1 was knocked out or downregulated, as occurs in bsr-d1 plants upon infection. We further found that OsMYB30 bound to and activated the promoters of 4-coumarate:coenzyme A ligase genes (Os4CL3 and Os4CL5) resulting in accumulation of lignin subunits G and S. This action led to obvious thickening of sclerenchyma cells near the epidermis, inhibiting M. oryzae penetration at the early stage of infection.Our study revealed novel components required for bsr-d1-mediated resistance and penetration-dependent immunity, and advanced our understanding of broad-spectrum disease resistance.