Characterization of a Nilaparvata lugens (Stal) brummer gene and analysis of its role in lipid metabolism
Characterization of a Nilaparvata lugens (Stal) brummer gene and analysis of its role in lipid metabolism
复制标题
褐飞虱 (Stal) brummer 基因的特征及其在脂质代谢中的作用分析
DOI：
10.1002/arch.21442
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发表时间：
2018
影响因子：
2.2
通讯作者：
Lu K
中科院分区：
文献类型：
--
作者：
Zhou Jinming;Yan Jing;You Keke;Yuan Zhineng;Zhou Qiang;Lu Kai;Chen Xia;Lu Kai;Zhou Q;Lu K
brummer（bmm）基因编码脂质储存液滴相关的三酰甘油（TAG）脂肪酶，其属于Brummer/Nutrin亚家族。这些酶水解脂类代谢中TAG中的酯键，并在昆虫能量稳态中起作用。在某些稻飞虱物种中，暴露于某些农业化学品会导致繁殖力增加，这必然涉及脂质代谢。然而，bmm在稻飞虱脂质储存和动员中的生物学作用尚未被研究。本研究克隆了褐飞虱（Nilaparvata lugens）bmm（Nlbmm）的开放阅读框（ORF），并进行了序列测定。Nlbmm基因的开放阅读框为1014 bp，编码338个氨基酸残基，含有patatin结构域，与其它昆虫的patatin结构域具有很大的进化保守性，在脂肪体中高表达，与其在脂质代谢中的作用一致。注射Nlbmm双链RNA（dsNlbmm）导致Nlbmm mRNA积累减少，但不影响与脂质合成相关的几个基因的表达，包括酰基辅酶A结合蛋白（ACBP）、乙酰辅酶A羧化酶（ACC）和载脂蛋白受体（LpR）。Nlbmm敲低导致全身TAG含量增加，总脂肪体脂质积累，Nlbmm基因敲低对甘油的合成和分布无影响。我们推测Nlbmm在N. lugens。
Thebrummer(bmm) genes encode the lipid storage droplet‐associated triacylglycerols (TAG) lipases, which belong to the Brummer/Nutrin subfamily. These enzymes hydrolyze the ester bonds in TAG in lipid metabolism and act in insect energy homeostasis. Exposure to some agricultural chemicals leads to increased fecundity, which necessarily involves lipid metabolism, in some planthopper species. However, the biological roles of bmm in planthopper lipid storage and mobilization have not been investigated. Here, the open reading frame (ORF) ofbmm(Nlbmm) was cloned and sequenced from the brown planthopper (BPH;Nilaparvata lugens). The ORF is 1014 bp encoding 338 amino acid residues.Nlbmm contained patatin domains and shared considerable evolutionary conservation with other insect bmms.Nlbmmis highly expressed in the fat body, consistent with its roles in lipid metabolism. Injection withNlbmmdouble‐stranded RNA (dsNlbmm) led to reducedNlbmmmRNA accumulation, but did not influence expression of several genes related to lipid synthesis including acyl‐CoA‐binding protein (ACBP), acetyl‐CoA carboxylase (ACC), and a lipophorin receptor (LpR).Nlbmmknockdown led to increased TAG contents in whole bodies, accumulation of total fat body lipid, and decreased hemolymph lipid content.Nlbmmknockdown did not influence the synthesis and distribution of glycerol. We infer thatNlbmm acts in TAG breakdown and fat metabolism inN. lugens.