Biomimicry, biomineralization, and bioregeneration of bone using advanced three-dimensional fibrous hydroxyapatite scaffold
Biomimicry, biomineralization, and bioregeneration of bone using advanced three-dimensional fibrous hydroxyapatite scaffold
复制标题
使用先进的三维纤维羟基磷灰石支架进行骨的仿生、生物矿化和生物再生
DOI：
10.1016/j.mtadv.2019.100014
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发表时间：
2019-09-01
影响因子：
10
通讯作者：
Li, J.
中科院分区：
文献类型：
--
作者：
Li, Z.;Chu, D.;Li, J.
仿生学、生物矿化和生物再生技术将为再生医学铺平道路，以改善人类健康。具有细胞外基质样天然结构和三维（3D）网络的功能性纤维支架对于满足细胞和新骨组织生长的复杂要求至关重要。在这项研究中，我们制作了三维纤维羟基磷灰石支架（3D FHAS）的仿生，生物矿化，生物再生功能，对损伤的骨组织的再生和修复的综合电纺丝，相变和矿化技术。我们的方法提高了纤维支架的成骨性能。我们的3D FHAS是从蚕丝纳米纤维中获得的，它允许羟基磷灰石（HA）层生长到满足骨骼生长需要的厚度范围。具有深度互连孔的3D纤维结构有助于细胞和营养进入内部，并将排泄物输送到外部，以实现出色的3D生长。此外，3D FHAS表面的微观结构与骨结构相似，易于HA被身体吸收和利用，从而促进新骨组织形成。体内外实验结果表明，与三维丝素纤维支架相比，我们的三维FHAS能显著提高骨髓间充质干细胞的成骨能力。(c)2019年，任作家。由爱思唯尔有限公司出版。这是一篇开放获取的文章，使用CC BY许可证（http：//creativecommons.org/licenses/by/4.0/）。
Biomimicry, biomineralization, and bioregeneration technologies will pave the way for regenerative medicine toward better human health. Functional fibrous scaffold with extracellular matrix-like natural structure and the three-dimensional (3D) network is crucial to meet the complex requirements of cell and new bone tissue growth. In this study, we fabricated 3D fibrous hydroxyapatite scaffold (3D FHAS) with biomimicry, biomineralization, and bioregeneration functionalities toward the regeneration and repair of injured bone tissues by integrated electrospinning, phase transformation, and mineralization technique. Our method improved the osteogenic property of the fibrous scaffold. Our 3D FHAS is obtained from silk nanofibers, and it allows the hydroxyapatite (HA) layer to grow into the thickness range that meets the needs of bone growth. The 3D fibrous structure with deeply interconnected pores facilitates cells and nutrition entry into the inside and excreta deliver outside to achieve excellent 3D growth. Moreover, the microstructure of the 3D FHAS surface is similar to the bone structure, easing the HA absorption and utilization by body and thus the new bone tissue formation. In vitro and in vivo results indicate that our 3D FHAS can greatly improve the osteogenesis of bone marrow mesenchymal stem cells as compared with that of 3D silk fibrous scaffolds. (c) 2019 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).