Immobilisation and biofilm development of Phanerochaete chrysosporium on polysulphone and ceramic membranes
Immobilisation and biofilm development of Phanerochaete chrysosporium on polysulphone and ceramic membranes
复制标题
黄孢原毛平革菌在聚砜和陶瓷膜上的固定化和生物膜形成
DOI：
10.1016/j.memsci.2005.04.014
复制
发表时间：
2005
影响因子：
9.5
通讯作者：
H. J. Small
中科院分区：
文献类型：
--
作者：
M. Sheldon;H. J. Small
在膜生物反应器的设计中，膜的形态是一个非常重要的方面，因为膜的开发或选择应该提供最大的表面积和理想的生物膜生长环境。对黄孢原毛平革菌(Phanerochaete Chrysosporium)的孢子固定化、生物膜形成和胞外酶产生膜进行了研究。分别鉴定了一种内壁毛细聚砜、两种平均孔径分别为0.2m和3μm的管状氧化铝陶瓷膜和一种平均孔径为3μm的毛细管二氧化钛陶瓷膜。孢子在毛细管膜上的附着和固定化程度最高，管状陶瓷膜上的次之。3μm毛细陶瓷膜的平均生物膜厚度为1140μm，3μm管状陶瓷膜的平均生物膜厚度为830μm，3μm管状陶瓷膜的平均生物膜厚度为400μm，而0.2μm管状陶瓷膜的平均生物膜厚度仅为40 Mpa m。聚砜和毛细陶瓷膜反应器均可连续生产LIP和MNP酶，管式陶瓷膜反应器未检测到LIP和MNP酶的活性。然而，对于反应器的日常运行，陶瓷膜更坚硬，机械稳定性更高，可以通过化学和蒸汽清洗重复使用。
In the design of a membrane bioreactor, the membrane morphology is a very important aspect, as the membrane should be developed or chosen to provide maximum surface area and an ideal environment for biofilm development. An investigation was carried out to identify membranes suitable for spore immobilisation, biofilm development and extracellular enzyme production from Phanerochaete chrysosporium. An internally skinned capillary polysulphone, two tubular aluminium oxide ceramic membranes with an average pore size of 0.2 and 3μm respectively and one capillary titanium oxide ceramic membrane with an average pore size of 3μm were identified and tested. The highest attachment and immobilisation of spores were observed on the capillary membranes with less on the tubular ceramic membranes. On average biofilm thicknesses of 1140μm were developed on the 3μm capillary ceramic membrane, 830μm on the capillary polysulphone membrane, 400μm on the 3μm tubular ceramic membrane with only 40μm on the 0.2μm tubular ceramic membrane. Continuous LiP and MnP enzyme production was achieved with both the polysulphone and capillary ceramic membrane reactors and no activity was detected with the tubular ceramic membrane reactors. However, for day-to-day operation of the reactor, the ceramic membranes were more rigid, mechanically stable and could be chemically and steam cleaned for re-use.