Inducement and Evaluation of a Murine Model of Experimental Myopia
Inducement and Evaluation of a Murine Model of Experimental Myopia
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DOI：
10.3791/58822
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发表时间：
2019-01-01
影响因子：
1.2
通讯作者：
Tsubota, Kazuo
中科院分区：
文献类型：
--
作者：
Jiang, Xiaoyan;Kurihara, Toshihide;Tsubota, Kazuo
由于基因操作相对容易，近视小鼠模型可以成为近视研究的有力工具。引起动物近视的一种方法是将透明负镜片放在眼前数周（镜片引起的近视，LIM）。然而，现有的诱导和评估方案因实验室而异。在这里，我们描述了一种高度实用且可重复的方法，使用新设计的眼镜在小鼠中诱导 LIM。该方法将晶状体稳定地固定在小鼠眼前，同时允许将晶状体取下进行清洁或局部给药。表型稳健、高效，且方差小。这里描述的方法可以应用于断奶后的小鼠，这延长了实验的可能持续时间。我们还提供了技术建议，以实现折射和轴长测量的可重复结果。我们希望这里描述的分步协议和详细的文章可以帮助研究人员更顺利地进行近视实验，并使数据在各个实验室之间具有可比性。
Murine model of myopia can be a powerful tool for myopia research because of the comparatively easy genetic manipulation. One way to induce myopia in animals is to put clear minus lenses in front of eyes for weeks (lens-induced myopia, LIM). However, extant protocols for inducement and evaluation vary from laboratory to laboratory. Here, we described a highly practical and reproducible method to induce LIM in mice using newly designed eyeglasses. The method fixes the lens stably in front of the mouse eye while allows the lens to be taken off for cleaning or topical drug administration. The phenotype is robust and efficient, and the variance is small. The method described here can be applied to mice right after weaning which extends the possible duration for experiments. We also gave technical advises for achieving reproducible results in refraction and axial length measurements. We hope the step-by-step protocol described here and the detailed article can help researchers perform myopia experiments with myopia more smoothly and make the data comparable across laboratories.