Label-free optical biosensor with microfluidics for sensing ligand-directed functional selectivity on trafficking of thrombin receptor
Label-free optical biosensor with microfluidics for sensing ligand-directed functional selectivity on trafficking of thrombin receptor
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DOI：
10.1016/j.febslet.2011.03.003
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发表时间：
2011-04-06
期刊：
影响因子：
3.5
通讯作者：
Fang, Ye
中科院分区：
文献类型：
--
作者：
Goral, Vasiliy;Wu, Qi;Fang, Ye
配体导向的功能选择性引起了人们对药物药理学分子描述和药物发现过程的极大兴趣。本文描述了一种用于全细胞传感的无标记光学生物传感器的发展。微流体控制激动剂暴露的持续时间和活化蛋白酶活化受体-1 （PAR(1)）的功能恢复。生物传感器表明，PAR(1)信号的持久性依赖于激动剂，而激动剂反过来又影响受体的再敏化过程。本研究展示了一种新的方法来区分配体对受体运输的功能选择性。(C) 2011年欧洲生化学会联合会。Elsevier B.V.版权所有。
Ligand-directed functional selectivity has stimulated much interest in the molecular delineation of drug pharmacology and the process of drug discovery. Here we describe the development of a label-free optical biosensor with microfluidics for whole cell sensing. The microfluidics controls the duration of agonist exposure and of the functional recovery of activated protease activated receptor-1 (PAR(1)). The biosensor manifests that the persistency of PAR(1) signaling is dependent on the agonists, which, in turn, affects the receptor resensitization process. This study demonstrates a new means to differentiate ligand-directed functional selectivity on trafficking of receptors. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.