Mature human spermatozoa do not transcribe novel RNA
Mature human spermatozoa do not transcribe novel RNA
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DOI：
10.1111/j.1439-0272.2005.00656.x
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发表时间：
2005-06-01
期刊：
影响因子：
2.4
通讯作者：
Anderegg, U
中科院分区：
文献类型：
--
作者：
Grunewald, S;Paasch, U;Anderegg, U
成熟精子含有以前在人类睾丸中发现的mRNA亚群。基于这一发现，假设精子的mRNA在精子发生过程中被转录。然而，到目前为止，还没有证据证明人类精子的转录活性。为了解决这个问题，我们进行了体外标记实验，放射性标记的尿苷三磷酸，然后分析细胞RNA。从人精子纯化的RNA中几乎没有检测到放射性RNA，证明成熟精子的转录活性。精子RNA显然是精子发生过程中转录的产物，可用于诊断目的。这些发现可能对不孕症患者具有诊断和治疗价值，因为精子RNA可能在受精后补充卵母细胞的RNA库。
Mature spermatozoa contain subsets of mRNA that have been found in human testes before. Based on this finding it was hypothesized that the mRNAs of spermatozoa are transcribed during spermatogenesis. However, up to now there is no proof of the transcriptional inactivity of human sperm. To address this issue we performed in vitro labelling experiments with radio-labelled uridine triphosphate followed by analysis of cellular RNA. There was virtually no radioactive RNA detectable in the RNA purified from human spermatozoa proving the transcriptional inactivity of mature spermatozoa. The spermatozoal RNA obviously results from transcription during spermatogenesis and can be used for diagnostic purposes. These findings might have diagnostic and - possibly - therapeutic value in infertility patients as spermatozoal RNAs might complement the RNA pool of the oocyte after fertilization.