Prostaglandin E2 enhances axonal transport and neuritogenesis in cultured mouse dorsal root ganglion neurons
Prostaglandin E2 enhances axonal transport and neuritogenesis in cultured mouse dorsal root ganglion neurons
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DOI：
10.1016/s0306-4522(00)00347-x
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发表时间：
2000-01-01
期刊：
影响因子：
3.3
通讯作者：
Kawakami, T
中科院分区：
文献类型：
--
作者：
Hiruma, H;Ichikawa, T;Kawakami, T
通过视频增强显微镜观察前列腺素E-2对培养小鼠背根神经节神经元轴突转运的影响。前列腺素E-2的应用增加了逆行和顺行方向运输的颗粒数量。EP2前列腺素受体激动剂butaprost可模拟前列腺素E-2的作用，而EP1/EP3前列腺素受体激动剂17-苯基三肽前列腺素E-2和EP3前列腺素受体激动剂M&B 28767则无作用。膜渗透性环AMP类似物二丁基环AMP和腺苷酸环化酶激活剂forskolin模拟前列腺素E-2的作用。蛋白激酶A抑制剂H-89可逆地减少了逆行和逆行方向的颗粒数量。H-89可阻断前列腺素E-2和二丁基环AMP的作用。结合先前的生化研究表明前列腺素E-2增加环AMP水平，本研究结果表明前列腺素E-2通过EP2受体和环AMP依赖性蛋白激酶A途径增强轴突转运。我们进一步研究了前列腺素E-2在神经突生长中的作用。前列腺素E-2增加了显示神经突的细胞数量和神经突生长速度，其作用机制与刺激轴突运输类似。前列腺素E-2可能调节轴突运输，为感觉神经元的形态发生和其他功能提供物质。(c) 2000 ibro。Elsevier Science Ltd.出版。版权所有。
The effects of prostaglandin E-2 on axonal transport in cultured mouse dorsal root ganglion neurons were investigated by analysing the number of axonally transported particles under video-enhanced microscopy. Application of prostaglandin E-2 increased the number of particles transported in anterograde and retrograde directions. The EP2 prostaglandin receptor agonist butaprost mimicked the effect of prostaglandin E-2, but the EP1/EP3 prostaglandin receptor agonist 17-phenyl trinor prostaglandin E-2 and the EP3 prostaglandin receptor agonist M&B 28767 had no effect. The membrane-permeable cyclic AMP analogue dibutyryl cyclic AMP and the adenylate cyclase activator forskolin mimicked the effect of prostaglandin E-2. The protein kinase A inhibitor H-89 reversibly reduced the number of particles in both anterograde and retrograde directions. The effects of prostaglandin E-2 and dibutyryl cyclic AMP were blocked by H-89. Taken together with previous biochemical studies showing that prostaglandin E-2 increases cyclic AMP levels, the present results suggest that prostaglandin E-2 enhances axonal transport via the EP2 receptor and cyclic AMP-dependent protein kinase A pathway. We further investigated the role of prostaglandin E-2 in neurite growth. Prostaglandin E-2 increased both the number of cells exhibiting neurites and the neurite growth rate, operating by a similar mechanism to stimulation of axonal transport.Prostaglandin E-2 may modulate axonal transport to supply materials for morphogenesis as well as other functions in sensory neurons. (C) 2000 IBRO. Published by Elsevier Science Ltd. All rights reserved.