Segmental Duplication of Chromosome 11 and its Implications for Cell Division and Genome-wide Expression in Rice.
Segmental Duplication of Chromosome 11 and its Implications for Cell Division and Genome-wide Expression in Rice.
复制标题
水稻 11 号染色体的片段复制及其对细胞分裂和全基因组表达的影响
DOI：
10.1038/s41598-017-02796-9
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发表时间：
2017-06-02
影响因子：
4.6
通讯作者：
Gong Z
中科院分区：
文献类型：
--
作者：
Zhang R;Xue C;Liu G;Liu X;Zhang M;Wang X;Zhang T;Gong Z
分段重复是在染色体中发生的主要结构变化。源自短染色体11（11L+11L+11s∙11s∙11S，“∙”代表着丝粒DNA重复位置），并分析了其对细胞分裂和基因组表达的含义，仅在11S∙11S的中心效果上是一个有效的。单中心然而，染色体。通常在等效平面上排列的结构三分化染色体，或者在减数分裂时影响其分离。 ARM 11S-2没有展示显然，剂量补偿，如1.00的峰值所示，基因剂量效应将减少一代人的性繁殖。
Segmental duplication is a major structural variation that occurs in chromosomes. Duplication leads to the production of gene copies with increased numbers of related repeat segments, causing the global genome to be in a state of imbalance. In addition, if the added segment contains a centromeric specific DNA, the duplicated chromosome will have structural multiple centromeres. We identified a segmental duplication containing structurally tricentric regions derived from the short arm of chromosome 11 (11L∙ + 11L∙ + 11S∙11S∙11S∙11S, “∙” represents the centromeric DNA repeat loci), and analyzed its implications for cell division and genome-wide expression. In the variant, only the middle centromere of 11S∙11S∙11S∙11S is functionally active. As a result, the structurally tricentric chromosome was stable in mitosis, because it is actually a functional monocentric chromosome. However, the structurally tricentric chromosome, which usually formed a bivalent, was either arranged on the equatorial plane or was lagging, which affected its separation during meiosis. Furthermore, RNA-seq and RT-qPCR analysis showed that the segmental duplication affected genome-wide expression patterns. 34.60% of genes in repeat region showed positive dosage effect. Thus, the genes on chromosome arm 11S-2 didn’t exhibit obviously dosage compensation, as illustrated by no peak around a ratio of 1.00. However, the gene dosage effect will reduce after sexual reproduction of a generation.