Roles of miR-278-3p in IBP2 regulation and Bombyx mori cytoplasmic polyhedrosis virus replication
Roles of miR-278-3p in IBP2 regulation and Bombyx mori cytoplasmic polyhedrosis virus replication
复制标题
miR-278-3p在IBP2调节和家蚕胞质多角体病毒复制中的作用
DOI：
10.1016/j.gene.2015.09.009
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发表时间：
2016
期刊：
影响因子：
3.5
通讯作者：
Guo Xijie
中科院分区：
文献类型：
--
作者：
Wu Ping;Qin Guangxing;Qian Heying;Chen Tao;Guo Xijie
家蚕胞质型多角体病毒（BmCPV）是家蚕的主要病毒性病原，对我国蚕业的发展是一个巨大的挑战。BmCPV感染诱导的胰岛素相关肽结合蛋白2（Insulin-related peptide binding protein 2，IBP 2）基因可能在BmCPV感染中起重要作用。免疫反应。microRNA（miRNAs）是一类在转录后水平调控基因表达的小分子非编码RNA，在免疫和抗病毒反应等过程中发挥重要作用。在本研究中，我们通过荧光素酶报告基因分析鉴定了IBP 2是miR-278- 3 p的靶点之一。miR-278- 3 p的过表达对家蚕幼虫IBP 2的表达具有负调控作用，对BmCPV的mRNA转录水平具有正调控作用。我们的研究结果表明，miR-278- 3 p可能在BmCPV复制中发挥重要作用。这是首次报道bmo-miRNAs在BmCPV感染应答中的作用，为探讨BmCPV感染和宿主免疫的分子机制提供了新的线索。
Bombyxmori cytoplasmic polyhedrosis virus (BmCPV) is a major viral pathogen of silkworm and remains a big challenge to the sericultural industry. Insulin-related peptide binding protein 2 (IBP2) gene, induced by BmCPV infection may play an important role inB. moriimmune response. MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression at the post-transcriptional level and play an important role in various processes, including immunity and antiviral response. In this study, we identified IBP2 as one of the targets for miR-278-3p by using luciferase reporter assay. Overexpression of miR-278-3p negatively regulates the expression of IBP2 in silkworm larvae and positively regulates the mRNA transcript level of BmCPV. Our results suggest that miR-278-3p may play an important role in BmCPV replication. It's the first report on bmo-miRNAs in response to BmCPV infection and could provide a new clue to explore the molecular mechanism of BmCPV infection and host immunity.