Genome-wide identification and comparative analysis of the TUBBY-like protein gene family in maize
Genome-wide identification and comparative analysis of the TUBBY-like protein gene family in maize
复制标题
DOI：
10.1007/s13258-015-0338-6
复制
发表时间：
2016-01-01
期刊：
影响因子：
2.1
通讯作者：
Ma Qing
中科院分区：
文献类型：
--
作者：
Chen Yulong;Dai Wei;Ma Qing
TLP含有高度保守的Tubby结构域，其进化历史可以追溯到真核生物进化的早期阶段，因为这个基因家族在从单细胞到多细胞的真核生物中都得到了鉴定。虽然最近在拟南芥和水稻中报道了对整个TLP基因家族的全基因组结构和进化分析，但对玉米中的TLP基因知之甚少。为了深入了解TLP基因是如何在玉米中进化的，我们对该作物中TLP基因的分子进化进行了全面的分析。通过全基因组筛选，共鉴定出15个玉米TLP基因(ZmTLP1-15)。根据系统发育关系、蛋白质结构域和基序组织，将该家族划分为四个亚家族。基因复制和染色体定位分析表明，节段性复制在玉米TLP家族的扩大中发挥了重要作用。ZmTLP基因在ABA、氯化钠、42℃、4℃和聚乙二醇胁迫处理下表现出差异表达。通过对含有TLP基因的特定区域的比较，我们对三种禾本科植物进行了微共线分析，揭示了玉米、水稻和高粱之间大量的微共线基因对，这表明TLP基因的侧翼区域可能来自一个共同的远古禾本科祖先。
The evolutionary history of TUBBY-like proteins (TLPs), which contain a highly conserved tubby domain, can be traced to the early stages of eukaryote evolution, on account of the identification of this gene family in organisms from single-celled to multicellular eukaryotes. While genome-wide structural and evolutionary analyses of the entire TLP gene family have recently been reported in Arabidopsis and rice, little is known about TLP genes in maize. To gain insight into how TLP genes have evolved in maize, we conducted comprehensive analysis of the molecular evolution of TLP genes in this crop. A total of 15 TLP genes (ZmTLP1-15) were identified in maize by genome-wide screening. This family was classified into four subfamilies based on phylogenetic relationships, protein domains, and motif organization. Gene duplication and chromosomal location analysis indicated that segmental duplication has played a major role in the expansion of the maize TLP family. The ZmTLP genes exhibited differential expression profiles under ABA, NaCl, 42, 4 degrees C, and PEG stress treatment. We performed microsynteny analysis across three gramineous species based on comparisons of the specific regions containing TLP genes, revealing numerous microsyntenic gene pairs among maize, rice, and sorghum, which suggests that the flanking regions of TLP genes may be derived from a common ancient Gramineae ancestor.