Quantitative determination of paraquat in meconium by sodium borohdride-nickel chloride chemical reduction and gas chromatography/mass spectrometry (GC/MS)
Quantitative determination of paraquat in meconium by sodium borohdride-nickel chloride chemical reduction and gas chromatography/mass spectrometry (GC/MS)
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DOI：
10.1016/j.jchromb.2007.11.002
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发表时间：
2008-02-01
影响因子：
3
通讯作者：
Bielawski, Dawn M.
中科院分区：
文献类型：
--
作者：
Posecion, Norberto C.;Ostrea, Enrique M.;Bielawski, Dawn M.
本研究的目的是开发一种用于GC/MS分析胎粪中百草枯作为胎儿接触百草枯的生物标志物的程序。该方法涉及硼氢化钠-氯化镍还原程序，液-液萃取的过氢化产物，浓缩，和GC/MS分析。该方法具有良好的总回收率（102.56%），%CV（批间）小于13%，检测限为0.0156 μ g/g。对菲律宾一个研究人群（n = 70）的胎粪样本进行的分析显示，胎儿接触百草枯的流行率为2.8%。(c)2007 Elsevier B. V.保留所有权利。
The objective of this study was to develop a procedure for the GC/MS assay of paraquat in meconium as a biomarker of fetal exposure to paraquat. The method involved a sodium borohydride-nickel chloride reduction procedure, liquid-liquid extraction of the perhydrogenated product, concentration, and GC/MS assay. The method demonstrated good overall recovery (102.56%) with %CV (inter-assay) of less than 13%, and a limit of detection of 0.0156 mu g/g. Analysis of meconium samples from a study population in the Philippines (n = 70) showed a 2.8% prevalence of fetal exposure to paraquat. (c) 2007 Elsevier B.V. All rights reserved.