The combinational use of CRISPR/Cas9-based gene editing and targeted toxin technology enables efficient biallelic knockout of the α-1,3-galactosyltransferase gene in porcine embryonic fibroblasts.
The combinational use of CRISPR/Cas9-based gene editing and targeted toxin technology enables efficient biallelic knockout of the α-1,3-galactosyltransferase gene in porcine embryonic fibroblasts.
复制标题
结合使用基于 CRISPR/Cas9 的基因编辑和靶向毒素技术,可以实现猪高效成纤维细胞中 α-1,3-半乳糖基转移胚胎酶基因的双等位基因敲除。
作者:
Masahiro Sato;Kazuchika Miyoshi;Yozo Nagao;Yohei Nishi;Masato Ohtsuka;Shingo Nakamura;Takayuki Sakurai;Satoshi Watanabe