A rapid, simple measurement of human albumin in whole blood using a fluorescence immunoassay (I)
A rapid, simple measurement of human albumin in whole blood using a fluorescence immunoassay (I)
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DOI：
10.1016/j.cccn.2003.10.002
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发表时间：
2004-01-01
影响因子：
5
通讯作者：
Oh, SW
中科院分区：
文献类型：
--
作者：
Choi, S;Choi, EY;Oh, SW
背景：人血清白蛋白(HSA)是体内含量最丰富的血浆蛋白，在代谢中起着关键作用。白蛋白浓度的变化为代谢性疾病和诊断应用提供了有价值的信息。方法：我们构建了两个白蛋白定量检测系统。该免疫分析采用荧光染料检测样品中的白蛋白，并采用常规层析作为分离系统。该检测系统由抗-HSA-mAb或固定在一次性试纸中的HSA、荧光标记的检测器缓冲液和激光荧光扫描仪组成。我们将样品与检测器混合，装入试剂盒，孵育10分钟，在激光荧光扫描仪中测量白蛋白浓度。我们使用日立747生化分析仪检测了与自动卡介苗染料结合法检测的可比性。结果：用相对荧光单位换算的A(T)/A(C)与白蛋白浓度的相关系数在竞争性和抑制性检测系统中均具有较好的可靠性(r=0.998)。使用Bland-Altman差图分析，我们观察到两种方法，即日立生化分析仪的荧光免疫层析法(FL-ICA)和自动卡介苗染料结合法，在HSA浓度的临床相关范围内具有可接受的一致性。免疫分析系统的批内和批间变异系数(CV)为
Background: Human serum albumin (HSA) is the most abundant plasma protein and plays key a role in metabolism. The variation in albumin concentration provides valuable information related to metabolic diseases and diagnostic application. Methods: We constructed two assay systems to quantify the albumin concentration. The immunoassay used a fluorescence (FL) dye to detect albumin in samples and employed the conventional chromatography as a separation system. The assay system consists of an anti-HSA-mAb or an HSA immobilized test strip in a disposable cartridge, a fluorescence-labeled detector buffer and a laser-fluorescence scanner. We mixed the sample with detector, loaded it onto a cartridge, incubated it for 10 min and measured the concentration of albumin in a laser-fluorescence scanner. We examined the comparability of assay with an automated BCG dye binding method using a Hitachi 747 biochemical analyzer. Results: The correlation of coefficient between A(T)/A(C) as converted from the relative fluorescence units (RFU) and albumin concentration displayed reasonable reliability in both the competition and the inhibition assay systems (r = 0.998). Using the Bland-Altman difference plot analysis, we observed an acceptable agreement between two methods, the fluorescence immunochromatography assay (FL-ICA) and the automated BCG dye-binding method of a Hitachi biochemical analyzer, over the clinical relevant range of HSA concentrations. The coefficient of variation (CV) of within- and between-run variation in the immunoassay system was