[Autoantibody diagnostics in neurology using native and recombinant antigenic substrates].
[Autoantibody diagnostics in neurology using native and recombinant antigenic substrates].
复制标题
[使用天然和重组抗原底物进行神经病学自身抗体诊断]。
DOI：
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
C. Probst
中科院分区：
文献类型：
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作者：
W. Stöcker;S. Saschenbrecker;K. Rentzsch;L. Komorowski;C. Probst
神经相关自身抗体的现代诊断是基于使用海马体、小脑和其他组织的组织切片的间接免疫荧光。对于单特异性检测，使用了转染了不同神经抗原的人胚胎肾(HEK)细胞。生物芯片马赛克的设计目的是提供快速概述，并包含20种或更多物质，它们与血清或脑脊液(CSF)样本孵育，在反应场上彼此相邻放置。此外，还使用基于小脑或海马体提取液的蛋白质印迹或含有已定义重组抗原的线条印迹。最初的研究应该总是包括所有主要的抗神经自身抗体的平行分析，而不是只进行单一参数测试。直到几年前，主要研究针对细胞内神经元抗原的自身抗体。然而，更常见的是针对神经细胞表面结构的抗体，特别是针对谷氨酸受体(NMDA型)的抗体。
Modern diagnostics for the determination of neurologically relevant autoantibodies are based on indirect immunofluorescence using tissue sections of the hippocampus, cerebellum and other tissues. For monospecific detection human embryonic kidney (HEK) cells transfected with different neurological antigens are used. Biochip mosaics are designed to give a quick overview and contain 20 or more substances positioned next to each other on a reaction field, which are incubated with the serum or cerebrospinal fluid (CSF) sample. Western blots based on cerebellum or hippocampus extracts or line blots containing defined recombinant antigens are used additionally. Initial investigations should always comprise the parallel analysis of all major antineural autoantibodies instead of performing only single parameter tests. Up until a few years ago autoantibodies against intracellular neuronal antigens were mainly investigated. Antibodies against structures of the neural cell surface, however, are much more frequently found, especially those against glutamate receptors (type NMDA).