THE GENE FOR THE RNA COMPONENT OF THE MITOCHONDRIAL RNA-PROCESSING ENDORIBONUCLEASE IS LOCATED ON HUMAN CHROMOSOME-9P AND ON MOUSE CHROMOSOME-4
THE GENE FOR THE RNA COMPONENT OF THE MITOCHONDRIAL RNA-PROCESSING ENDORIBONUCLEASE IS LOCATED ON HUMAN CHROMOSOME-9P AND ON MOUSE CHROMOSOME-4
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DOI：
10.1016/0888-7543(90)90483-b
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发表时间：
1990-03-01
期刊：
影响因子：
4.4
通讯作者：
FRANCKE, U
中科院分区：
文献类型：
--
作者：
HSIEH, CL;DONLON, TA;FRANCKE, U
线粒体 RNA 加工内切核糖核酸酶 (RNAase MRP) 能够在独特位点切割与置换环轻链互补的线粒体 RNA。该酶是一种核糖核蛋白，其RNA成分是核基因产物。初级转录物的5''侧翼区具有RNA聚合酶II转录特征的控制元件，编码区具有RNA聚合酶III转录信号的特征。与 RNAase MRP 相关的 RNA 是第一个已知的由细胞核中的单拷贝基因编码的 RNA，并且被认为被输入到线粒体中。通过对 11 次人类的 Southern 印迹分析，将 RNAase MRP 的该 RNA 成分的基因 (RMRP) 分配给人类 9 号染色体和小鼠 4 号染色体。啮齿动物杂交和 11 只小鼠。啮齿类动物分别与探针 pHM1.0 和探针 pSP270 杂交。探针 pHSTU300 与正常人类染色体的原位杂交显示，100 个细胞中有 29 个在 9p 上有标记，302 个银粒中有 9.6% 位于 9p21-p12。
Mitochondrial RNA-processing endoribonuclease (RNAase MRP) has the capacity to cleave mitochondrial RNA complementary to the light strand of the displacement loop at a unique site. The enzyme is a ribonucleoprotein whose RNA component is a nuclear gene product. The 5'' flanking region of the primary transcript has control elements characteristic of RNA polymerase II transcription, and the coding region has features of RNA polymerase III transcription signals. The RNA associated with RNAase MRP is the first known RNA encoded by a single-copy gene in the nucleus and believed to be imported into mitochondria. The gene (RMRP) for this RNA component of RNAase MRP was assigned to human chromosome 9 and mouse chromosome 4 by Southern blot analyses of 11 human .times. rodent hybrids and 11 mouse .times. rodent hybrids with probe pHM1.0 and probe pSP270, respectively. In situ hybridization of probe pHSTU300 to normal human chromosomes revealed 29 of 100 cells with label on 9p and 9.6% of 302 silver grains located at 9p21-p12.