In vivo detecting mouse persistent hyperplastic primary vitreous by Spectralis Optical Coherence Tomography
In vivo detecting mouse persistent hyperplastic primary vitreous by Spectralis Optical Coherence Tomography
复制标题
通过 Spectralis 光学相干断层扫描在体内检测小鼠持续性增生性原发性玻璃体
DOI：
10.1016/j.exer.2019.02.017
复制
发表时间：
2019-04-01
影响因子：
3.4
通讯作者：
Zhao, Ling
中科院分区：
文献类型：
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作者：
Lian, Qing;Zhao, Minglei;Zhao, Ling
目的 通过 Spectralis 光学相干断层扫描 (OCT) 识别小鼠持续性增生性初级玻璃体 (PHPV) 的成像特征，并评估和比较 OCT 与彩色摄影 (CP) 和眼底荧光素血管造影 (FFA) 成像检测小鼠 PHPV 的灵敏度和精确度。本研究检查了年龄从 3 个月至 7 个月的 Notch4(-/-) C57BL/6J 小鼠（224 只眼睛）。利用CP、FFA和OCT成像来检查小鼠眼睛的玻璃体腔和视网膜。水平和径向 OCT 扫描体积以视神经乳头为中心。进行苏木精和伊红 (H&E) 染色以验证 PHPV。彩色摄影和FFA成像，在33只眼睛的玻璃体腔内有或没有血管中发现了晶状体后不规则的纤维血管膜样组织。其中，31 只眼睛使用 Spectralis OCT 观察到玻璃体腔内有横向和倾斜线性高反射混浊。还通过 OCT 测量了 PHPV 在眼后段的位置。小鼠 PHPV 通过 H&E 染色进行验证。使用 Spectralis OCT 在 PHPV 小鼠中检测到玻璃体腔中典型的高反射性混浊。 Spectralis OCT 成像可以像彩色摄影和 FFA 一样有效地检测小鼠 PHPV。
To identify imaging characteristics of mouse persistent hyperplastic primary vitreous (PHPV) by Spectralis Optical Coherence Tomography (OCT), as well as to assess and compare the sensitivity and precision of OCT with color photography (CP) and Fundus Fluorescein Angiography (FFA) imaging in detecting mouse PHPV. Notch4(-/-) C57BL/6J mice (224 eyes) aged from 3 months to 7 months were examined in this study. CP, FFA and OCT imaging were utilized to examine vitreous cavity and retina of mouse eyes. Horizontal and radial OCT scan volume was centered on the optic nerve head. Hematoxylin and eosin (H&E) staining was performed to validate PHPV. For color photography and FFA imaging, retrolental irregular fibrovascular membrane-like tissues were found in 33 eyes with/without blood vessels in vitreous cavity. Among them, 31 eyes were visualized with lateral and oblique linear hyperreflective opacities in vitreous cavity using Spectralis OCT. Position of PHPV in posterior segment of eyes was also measured via OCT. Mouse PHPV was validated by H&E staining. Typical hyperreflective opacities in vitreous cavity were detected in PHPV mouse using Spectralis OCT. Spectralis OCT imaging can effectively detect mouse PHPV as color photography and FFA.