Swine infection with H9N2 influenza viruses in China in 2004
Swine infection with H9N2 influenza viruses in China in 2004
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DOI：
10.1007/s11262-008-0227-z
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发表时间：
2008-06-01
期刊：
影响因子：
1.6
通讯作者：
Liu, Jin-Hua
中科院分区：
文献类型：
--
作者：
Cong, Yan-Long;Wang, Chun-Feng;Liu, Jin-Hua
2004年，在中国河南省的商业养猪场从患有呼吸综合征的猪中分离到H9 N2甲型流感病毒。对7株猪H9 N2流感病毒进行了抗原性和遗传学鉴定。猪源H9 N2病毒的血凝素抗原性与我国流行的禽源H9 N2病毒A/duck/Hong Kong/Y280/1997（Dk/HK/Y280/97）相似。值得注意的是，这些分离物的神经氨酸酶在茎部没有缺失，这在Dk/HK/Y280/97-like亚系中很少观察到。基因分析表明，7株分离株HA裂解位点均具有一个-R-S-S-R-基序，与我国禽源Dk/HK/Y280/97类病毒的基序一致。系统进化分析表明，7株H9 N2亚型猪源病毒均为Dk/HK/Y280/97亚型禽流感病毒。目前的结果表明，H9 N2病毒的禽-猪种间传播在中国继续存在，通过2004年，因此，猪流感的监测应给予高度重视。
In 2004, H9N2 influenza A viruses were isolated from pigs with respiratory syndrome in commercial swine farms in Henan province, China. Antigenic and genetic characterization were performed for seven swine H9N2 influenza viruses. The hemagglutinin antigenicity of swine H9N2 viruses was similar to those of avian H9N2 viruses of A/duck/Hong Kong/Y280/1997 (Dk/HK/Y280/97)-like sublineage prevalent in China. It is noteworthy that the neuraminidase of these isolates had no deletions in the stalk, which was seldom observed in those viruses of Dk/HK/Y280/97-like sublineage. Genetic analysis revealed that all seven isolates had an -R-S-S-R- motif at the HA cleavage site, which was the same as those of Dk/HK/Y280/97-like viruses established in avian population in China. Phylogenetic analyses showed that the seven swine H9N2 viruses were completely derived from avian influenza viruses of Dk/HK/Y280/97-like sublineage. The present results indicated that avian-to-pig interspecies transmission of H9N2 viruses continued to exist in China through 2004; therefore, surveillance of swine influenza should be given a high priority.