Identification of a novel member of yeast mitochondrial Hsp70-associated motor and chaperone proteins that facilitates protein translocation across the inner membrane
Identification of a novel member of yeast mitochondrial Hsp70-associated motor and chaperone proteins that facilitates protein translocation across the inner membrane
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DOI：
10.1016/j.febslet.2004.12.018
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发表时间：
2005-01-17
期刊：
影响因子：
3.5
通讯作者：
Endo, T
中科院分区：
文献类型：
--
作者：
Yamamoto, H;Momose, T;Endo, T
在这里，我们报道了酵母15-kD Tim15/Zim17的鉴定，这是线粒体Hsp70 （mtHsp70）相关马达和伴侣蛋白（MMC）的新成员。15-kD MMC蛋白是一种具有锌指基序的外周内膜蛋白。在体内和体外，15-kD蛋白的缺失导致含有序列的蛋白进入基质的功能受损。15-kD蛋白的过表达挽救了ssc1-3细胞中mtHsp70的功能缺陷，并且含有15-kD蛋白的融合蛋白与纯化的mtHsp70物理相互作用。因此，Tim15/Zim17与mtHsp70合作，促进含有序列的蛋白进入基质。(C) 2004年欧洲生化学会联合会。Elsevier B.V.版权所有。
Here, we report the identification of yeast 15-kD Tim15/Zim17, a new member of mitochondrial Hsp70 (mtHsp70)-associated motor and chaperone (MMC) proteins. The 15-kD MMC protein is a peripheral inner membrane protein with a zinc-finger motif. Depletion of the 15-kD protein led to impaired import of presequence-containing proteins into the matrix in vivo and in vitro. Overexpression of the 15-kD protein rescued the functional defects of mtHsp70 in ssc1-3 cells, and a fusion protein containing the 15-kD protein physically interacts with purified mtHsp70. Tim15/Zim17 therefore cooperates with mtHsp70 to facilitate import of presequence-containing proteins into the matrix. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.