S-Layered Aneurinibacillus and Bacillus spp. are susceptible to the lytic action of Pseudomonas aeruginosa membrane vesicles
S-Layered Aneurinibacillus and Bacillus spp. are susceptible to the lytic action of Pseudomonas aeruginosa membrane vesicles
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DOI：
10.1128/jb.180.9.2306-2311.1998
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发表时间：
1998-05-01
影响因子：
3.2
通讯作者：
Beveridge, TJ
中科院分区：
文献类型：
--
作者：
Kadurugamuwa, JL;Mayer, A;Beveridge, TJ
当具有不同晶格类型和晶格常数以及S-（糖）蛋白化学的S-层的嗜热脂肪芽孢杆菌和嗜热需氧动脉瘤芽孢杆菌的S-层菌株以及同基因无S-层变体在平板上的噬菌斑测定或细胞悬浮液上的CFU测量期间经受来自铜绿假单胞菌的膜囊泡（MV）时，所有细菌类型裂解。负染色，薄切片，和免疫金标记的MV制剂的电子显微镜显示，囊泡粘附在所有细菌表面，打破开放，并消化所有细胞类型的含肽聚糖的细胞壁。重组的S-层似乎不受MV的影响，十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析表明，S-（糖）蛋白保持完整，在MV攻击后的所有培养上清液中发现了作为肽聚糖分解产物的meso-二氨基庚二酸。这些结果表明，即使MV比穿透这些蛋白质阵列的通道大得多，革兰氏阳性菌上的S层不形成抵抗MV裂解作用的防御屏障。主要的攻击方式是通过从hn's释放肽聚糖水解酶，其穿透S层以消化下面的含肽聚糖的细胞壁。S层不受MV蛋白酶的影响。
When S-layered strains of Bacillus stearothermophilus and Aneurinibacillus thermoaerophilus, possessing S-layers of different lattice type and lattice constant as well as S-(glyco)protein chemistry, and isogenic S-layerless variants were subjected to membrane vesicles (MVs) from P. aeruginosa during plaque assays on plates or CFU measurements on cell suspensions, all bacterial types lysed. Electron microscopy of negative stains, thin sections, and immunogold-labelled MV preparations revealed that the vesicles adhered to all bacterial surfaces, broke open, and digested the underlying peptidoglycan-containing cell wall of all cell types. Reassembled S-layer did not appear to be affected by MVs, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that the S-(glyco)proteins remained intact, meso-Diaminopimelic acid, as a peptidoglycan breakdown product, was found in all culture supernatants after MV attack These results suggest that even though MVs are much larger than the channels which penetrate these proteinaceous arrays, S-layers on gram-positive bacteria do not form a defensive barrier against the lytic action of MVs. The primary mode of attack is by the liberation from the hn's of a peptidoglycan hydrolase, which penetrates through the S-layer to digest the underlying peptidoglycan-containing cell wall. The S-layer is not affected by MV protease.