DEVELOPMENT OF CYTOCHROME-P450 AROMATASE MESSENGER-RNA LEVELS AND ENZYME-ACTIVITY IN OVARIES OF NORMAL AND HYPOGONADAL (HPG) MICE
DEVELOPMENT OF CYTOCHROME-P450 AROMATASE MESSENGER-RNA LEVELS AND ENZYME-ACTIVITY IN OVARIES OF NORMAL AND HYPOGONADAL (HPG) MICE
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DOI：
10.1677/jme.0.0140295
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发表时间：
1995-06-01
影响因子：
3.5
通讯作者：
OSHAUGHNESSY, PJ
中科院分区：
文献类型：
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作者：
GRAY, SA;MANNAN, MA;OSHAUGHNESSY, PJ
细胞色素P450芳香酶(P450(Arom))是雄激素生物转化为雌激素所必需的。本研究对正常小鼠和缺乏循环促性腺激素的性腺功能低下(HPG)小鼠的卵巢P450(Arom)mRNA和酶活性进行了检测。采用半定量逆转录聚合酶链式反应(RT-PCR)技术检测细胞色素P450(AROM)基因表达水平，并直接测定芳香酶活性。RT-PCR检测到P450(Arom)基因在成年小鼠卵巢、子宫、肾脏、脑和骨骼肌中均有表达，但在心肌中未见表达。在正常小鼠的卵巢中，P450(Arom)mRNA在出生当天(第1天)可检测到，并在第15天显著升高，以第1~5天变化最显著。芳香酶活性在所有年龄段的卵巢中也可检测到，并在第1~7天显著增加。在HPG小鼠的卵巢中，第1天P450(Arom)mRNA表达正常，但到第15天P450(Arom)mRNA水平没有显著变化。这些结果表明，在只含原始卵泡的新生小鼠卵巢中，P450mRNA的基础表达不依赖于促性腺激素。然而，在1天后，卵巢P450(AROM)的正常表达需要促性腺激素，这与初级和次级卵泡的发育相一致。
The cytochrome P450 aromatase (P450(arom)) enzyme is required for bioconversion of androgen to oestrogen. In this study ovarian P450(arom) mRNA and enzyme activity have been measured during development in normal mice and hypogonadal (hpg) mice which lack circulating gonadotrophins. A semi-quantitative reverse transcription-PCR (RT-PCR) technique was used to measure cytochrome P450(arom) mRNA levels and aromatase enzyme activity was measured directly. Using RT-PCR, P450(arom) mRNA was detectable in the adult mouse ovary and also in the uterus, kidney, brain and skeletal muscle but not in cardiac smooth muscle. In the normal mouse, P450(arom) mRNA was detectable in the ovary on the day of birth (day 1) and levels increased significantly up to day 15 with the most marked changes seen between days 1 and 5. Aromatase activity was also detectable at all ages in the ovary and increased significantly between days 1 and 7. In ovaries from hpg mice, normal levels of P450(arom) mRNA were present on day 1 but there P450 was no significant change in P450(arom) mRNA at later ages up to day 15. These results show that in the newborn mouse ovary, which contains only primordial follicles, there is a basal expression of P450 mRNA which is not gonadotrophin-dependent. After 1 day, however, gonadotrophins are required for normal expression of ovarian P450(arom) and this coincides with development of primary and secondary follicles.