The detection of human sapoviruses with universal and genogroup-specific primers
The detection of human sapoviruses with universal and genogroup-specific primers
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DOI：
10.1007/s00705-006-0820-1
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发表时间：
2006-12-01
影响因子：
2.7
通讯作者：
Shinozaki, K.
中科院分区：
文献类型：
--
作者：
Okada, M.;Yamashita, Y.;Shinozaki, K.
萨普病毒(SV)引起人类胃肠炎，由基因分化的病毒组成。建立了针对衣壳蛋白编码区的套式逆转录聚合酶链式反应(RT-PCR)。通过对扩增产物的遗传分析，我们可以确定病毒的基因类型。此外，还开发了基因组特异的引物，根据基因组的不同，扩增出不同长度的扩增子。这些基因组特异的引物也被用作套式聚合酶链式反应的内引子。这两种简单的RT-PCR方法是检测和研究人类疱疹病毒的有力工具。
Sapovirus (SV) causes gastroenteritis in humans and comprises genetically divergent viruses. A nested reverse transcription-polymerase chain reaction (RT-PCR) targeting the capsid-protein-coding region was developed using universal and genogroup-specific primer sets. The universal primers were capable of detecting human SV genogroups I, II, IV and V. Genetic analysis of the amplified products enabled us to phylogenetically determine the genotypes of the viruses. In addition, genogroup-specific primers that amplified different lengths of the amplicon depending on the genogroup were developed. These genogroup-specific primers were also used as inner primers for the nested PCR. These two simple RT-PCR methods are powerful tools for both detection and epidemiological studies of human SV.