Effect of the PA-MSHA vaccine on septic serum-induced inflammatory response
Effect of the PA-MSHA vaccine on septic serum-induced inflammatory response
复制标题
PA-MSHA 疫苗对脓毒症血清诱导的炎症反应的影响
DOI：
10.3892/mmr.2013.1337
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发表时间：
2013-04-01
影响因子：
3.4
通讯作者：
Yao, Hong-Liang
中科院分区：
文献类型：
--
作者：
Liu, Xiang-Feng;Wang, Li;Yao, Hong-Liang
脓毒症是由严重感染引起的一种复杂的临床综合征，随后是放大和失调的炎症反应。尽管临床上取得了很大的进展，但这种复杂综合征的发病率和死亡率仍然很高。已证明从革兰氏阴性菌铜绿假单胞菌（PA-MSHA）的外膜蛋白衍生的疫苗表现出免疫调节特性。在本研究中，测定了PA-MSHA疫苗对脓毒症患者血清诱导的外周血单核细胞炎症反应的影响。观察到PA-MSHA预处理抑制脓毒血清诱导的肿瘤坏死因子-α的产生。此外，PA-MSHA治疗增加白细胞介素-10水平，并促进CD 4（+）CD 25（+）Foxp 3（+）T细胞的生成。因此，本研究的结果提供了与PA-MSHA在脓毒症治疗中的潜在应用相关的机制见解。
Sepsis is defined as a complex clinical syndrome caused by a serious infection followed by an amplified and deregulated inflammatory response. The complex syndrome is associated with a high rate of morbidity and mortality, despite substantial clinical advances. A vaccine derived from the outer membrane proteins of the Gram-negative bacteria Pseudomonas aeruginosa (PA-MSHA) has been demonstrated to exhibit immune modulatory properties. In the present study, the effect of the PA-MSHA vaccine on the inflammatory response induced by serum from septic patients in peripheral blood mononuclear cells was determined. It was observed that PA-MSHA pretreatment inhibits the production of septic serum-induced tumor necrosis factor-alpha. In addition, PA-MSHA treatment increases interleukin-10 levels and promotes the generation of CD4(+)CD25(+)Foxp3(+) T cells. Thus, the results of the current study provide mechanistic insight relevant to the potential application of PA-MSHA in the treatment of sepsis.