Hybrid hydroxyapatite nanoparticle colloidal gels are injectable fillers for bone tissue engineering.
Hybrid hydroxyapatite nanoparticle colloidal gels are injectable fillers for bone tissue engineering.
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DOI：
10.1089/ten.tea.2013.0075
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发表时间：
2013-08
影响因子：
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通讯作者：
Qun Wang;Z. Gu;Syed A Jamal;M. Detamore;C. Berkland
中科院分区：
文献类型：
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作者：
Qun Wang;Z. Gu;Syed A Jamal;M. Detamore;C. Berkland
可注射骨填充物已经成为治疗骨缺损所需的侵入性手术的替代方案。目前的骨填充物可能受益于动态性能的改善，如注射过程中的剪切变薄和放置后材料硬度的恢复。将带负电荷的无机羟基磷灰石纳米粒子与带正电荷的有机聚(d，L-乳酸-羟基乙酸共聚)纳米粒子组装在一起，形成了具有凝聚力的胶体凝胶。这种材料通过静电力保持在一起，而静电力可能会被剪切破坏，从而促进挤出、成型或注射。干燥的胶体凝胶的扫描电子显微镜显示出组织良好的三维多孔结构。流变学测试表明，某些胶体凝胶在剪切后可以恢复。当人脐带间充质干细胞种植在胶体凝胶上时，也是高度存活的。HAP/PLGA-NP胶体凝胶为骨组织的可注射性填充和再生提供了一种有吸引力的方案。
Injectable bone fillers have emerged as an alternative to the invasive surgery often required to treat bone defects. Current bone fillers may benefit from improvements in dynamic properties such as shear thinning during injection and recovery of material stiffness after placement. Negatively charged inorganic hydroxyapatite (HAp) nanoparticles (NPs) were assembled with positively charged organic poly(d,l-lactic-co-glycolic acid) (PLGA) NPs to create a cohesive colloidal gel. This material is held together by electrostatic forces that may be disrupted by shear to facilitate extrusion, molding, or injection. Scanning electron micrographs of the dried colloidal gels showed a well-organized, three-dimensional porous structure. Rheology tests revealed that certain colloidal gels could recover after being sheared. Human umbilical cord mesenchymal stem cells were also highly viable when seeded on the colloidal gels. HAp/PLGA NP colloidal gels offer an attractive scheme for injectable filling and regeneration of bone tissue.