Glucocorticoids dose-dependently increase plasma levels of granulocyte colony stimulating factor in man.
Glucocorticoids dose-dependently increase plasma levels of granulocyte colony stimulating factor in man.
复制标题
糖皮质激素剂量依赖性地增加人类粒细胞集落刺激因子的血浆水平。
DOI：
10.1210/jcem.83.3.4802
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Stylianos Kapiotis
中科院分区：
文献类型：
--
作者：
Bernd Jilma;P. Stohlawetz;T. Pernerstorfer;Hans;Claudia Müllner;Stylianos Kapiotis
糖皮质激素通过减少中性粒细胞的边缘化和从骨髓池中动员中性粒细胞来增加中性粒细胞计数。然而，这些作用的机制尚未完全阐明，但已证明地塞米松在体外增强集落刺激因子（G-CSF）的释放。因此，我们假设，地塞米松可能会增加G-CSF的血浆水平。在9名健康男性中进行了双盲、随机、安慰剂对照、三向交叉试验。每例受试者在3个观察期（各48小时）内接受4次相同的生理盐水、0.04 mg/kg或1.0 mg/kg地塞米松输注。低剂量地塞米松使24小时时的G-CSF水平较基线15.5 ng/L（CI：10.6-20.4）升高240%（CI：115-366%）。24小时时，高剂量地塞米松使G-CSF水平较基线12.3 ng/L（CI：9.7-14.9）升高871%（CI：592-1149%）（所有比较p=0.008）。在48小时时未观察到进一步增加，但效果不太明显（对于高剂量和低剂量地塞米松，分别为p<0.008和p=0.08）。所有受试者的粒细胞-巨噬细胞-CSF（GM-CSF）水平均低于检测限0.36 ng/L。总之，地塞米松剂量依赖性地增加健康男性的G-CSF水平，这可能是其对中性粒细胞的一些影响的原因。
Glucocorticoids increase neutrophil counts by decreasing the margination of neutrophils and mobilizing neutrophils from the bone marrow pool. The mechanisms for these effects however are not fully elucidated, but it has been demonstrated that dexamethasone enhances release of colony stimulating factor (G-CSF) in-vitro. We therefore hypothesized, that dexamethasone may increase plasma levels of G-CSF. A double blind, randomized, placebo-controlled, three-way cross-over trial was conducted in nine healthy men. Every subject received four identical infusions of saline, 0.04 mg/kg or 1.0 mg/kg dexamethasone during three observation periods of 48 hours each. The low dose of dexamethasone increased G-CSF levels from a baseline of 15.5 ng/L (CI: 10.6-20.4) by 240% (CI: 115-366%) at 24 hours. The high dexamethasone dose increased G-CSF levels from a baseline of 12.3 ng/L (CI: 9.7-14.9) by 871% (CI: 592-1149%) at 24 hours (p=0.008 for all comparisons). No further increase was observed at 48 hours but the effect was less pronounced (p<0.008 and p=0.08 for the high and the low dose of dexamethasone, respectively). Granulocyte-macrophage-CSF (GM-CSF) levels were below the assay's detection limit of 0.36 ng/L in all subjects. In conclusion, dexamethasone dose dependently increases G-CSF levels in healthy men, an effect which may account for some of its effects on neutrophils.