Analysis of the fifth cell cycle of mouse development.
Analysis of the fifth cell cycle of mouse development.
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小鼠发育第五细胞周期的分析。
DOI：
10.1530/jrf.0.0840029
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
J. Chisholm
中科院分区：
文献类型：
--
作者：
J. Chisholm
分析小鼠发育的第5个细胞周期，以确定每个细胞周期阶段的长度。在整个细胞周期的不同时间，通过显微光密度测定法测量Feulgen染色的卵裂球核的DNA含量。为了实现第5个细胞周期开始的精确定时，实验利用分离的16-细胞卵裂球和通过分离的8-细胞卵裂球的体外分裂获得的细胞对。对极性和非极性16细胞卵裂球的混合群体进行了以下估计：G1，小于或等于2 h; S，8-9 h; G2 + M，2 h。极性和非极性细胞之间或细胞对和整个胚胎之间的DNA合成的时间没有显着差异。
The 5th cell cycle of mouse development was analyzed to determine the lengths of each cell cycle phase. The DNA content of Feulgen-stained blastomere nuclei was measured at various times throughout the cell cycle by microdensitometry. To achieve precise timing of the start of the 5th cell cycle, experiments utilized isolated 16-cell blastomeres and cell pairs obtained by in-vitro division of isolated 8-cell blastomeres. The following estimates were made for a mixed population of polar and apolar 16-cell blastomeres: G1, less than or equal to 2 h; S, 8-9 h; G2 + M, 2 h. No significant difference was found in the timing of DNA synthesis between polar and apolar cells or between cell pairs and whole embryos.