Multispectral Opto-acoustic Tomography (MSOT) of the Brain and Glioblastoma Characterization
Multispectral Opto-acoustic Tomography (MSOT) of the Brain and Glioblastoma Characterization
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DOI：
10.1016/j.neuroimage.2012.09.053
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发表时间：
2013-01-15
期刊：
影响因子：
5.7
通讯作者：
Ntziachristos, Vasilis
中科院分区：
文献类型：
--
作者：
Burton, Neal C.;Patel, Manishkumar;Ntziachristos, Vasilis
脑研究在很大程度上依赖于成像来评估体内的功能和疾病。我们在此研究多光谱光声断层扫描（MSOT），一种用于组织内部高分辨率分子成像的新技术。MSOT用多个波长的光脉冲照射组织，并检测吸收分子周围环境的热弹性膨胀产生的声波。通过对收集到的数据进行光谱分解分析，MSOT可以区分含氧和脱氧血红蛋白以及光吸收剂的光谱特征，并量化它们的浓度。由于能够检测到组织中几厘米深的吸收分子，它代表了小动物脑成像的理想模式，同时提供解剖学，血流动力学，功能和分子信息。在这项工作中，我们研究了MSOT在小鼠横断面脑成像中的能力。我们发现在小鼠大脑解剖和生理参数的横断面可视化中，光学成像性能是前所未有的。例如，通过使用二氧化碳挑战，证明了MSOT表征脑缺血区域的潜力。此外，静脉注射吲哚菁绿（ICG），并实时观察其在脑血管中的摄取和清除动力学。我们进一步发现，将U87肿瘤细胞注射到大脑中，可以通过完整的小鼠头部进行多参数、多光谱成像，例如通过肿瘤生长中的脱氧血红蛋白可视化。我们还展示了MSOT如何为大脑研究提供了几个引人注目的功能，并允许对大脑参数进行随时间的检测和量化，这些参数是使用其他无创成像方法无法获得的。(C) 2012年Elsevier Inc.出版。
Brain research depends strongly on imaging for assessing function and disease in vivo. We examine herein multispectral opto-acoustic tomography (MSOT), a novel technology for high-resolution molecular imaging deep inside tissues. MSOT illuminates tissue with light pulses at multiple wavelengths and detects the acoustic waves generated by the thermoelastic expansion of the environment surrounding absorbing molecules. Using spectral unmixing analysis of the data collected, MSOT can then differentiate the spectral signatures of oxygenated and deoxygenated hemoglobin and of photo-absorbing agents and quantify their concentration. By being able to detect absorbing molecules up to centimeters deep in the tissue it represents an ideal modality for small animal brain imaging, simultaneously providing anatomical, hemodynamic, functional, and molecular information. In this work we examine the capacity of MSOT in cross-sectional brain imaging of mice. We find unprecedented optical imaging performance in cross-sectional visualization of anatomical and physiological parameters of the mouse brain. For example, the potential of MSOT to characterize ischemic brain areas was demonstrated through the use of a carbon dioxide challenge. In addition, indocyanine green (ICG) was injected intravenously, and the kinetics of uptake and clearance in the vasculature of the brain was visualized in real-time. We further found that multiparameter, multispectral imaging of the growth of U87 tumor cells injected into the brain could be visualized through the intact mouse head, for example through visualization of deoxygenated hemoglobin in the growing tumor. We also demonstrate how MSOT offers several compelling features for brain research and allows time-dependent detection and quantification of brain parameters that are not available using other imaging methods without invasive procedures. (C) 2012 Published by Elsevier Inc.