Scale invariance of cell size fluctuations in starving bacteria
Scale invariance of cell size fluctuations in starving bacteria
复制标题
饥饿细菌细胞大小波动的尺度不变性
DOI：
10.1038/s42005-021-00739-5
复制
发表时间：
2021
影响因子：
5.5
通讯作者：
Takeuchi Kazumasa A.
中科院分区：
文献类型：
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作者：
Shimaya Takuro;Okura Reiko;Wakamoto Yuichi;Takeuchi Kazumasa A.
在稳定的环境中，细胞大小的波动被认为是由简单的物理原理，如最近发现的标度特性。在这里，通过开发一个微流控装置和使用E。大肠杆菌，我们调查的细胞大小波动对饥饿的反应。通过突然切换到无营养培养基，我们发现细胞大小分布发生变化，但满足标度不变性：重新标度的分布保持不变，并由饥饿前的生长条件决定。这些发现得到了基于细胞生长和细胞周期的模型的支持。此外，我们数值确定的范围内的规模不变性的有效性在不同的特征时间的饥饿过程中，并找到违反的规模不变性缓慢饥饿。我们的研究结果，结合理论论证，表明多叉复制的相关性，这有助于保留细胞周期状态的信息，从而可能导致规模不变性。
In stable environments, cell size fluctuations are thought to be governed by simple physical principles, as suggested by recent findings of scaling properties. Here, by developing a microfluidic device and usingE. coli, we investigate the response of cell size fluctuations against starvation. By abruptly switching to non-nutritious medium, we find that the cell size distribution changes but satisfies scale invariance: the rescaled distribution is kept unchanged and determined by the growth condition before starvation. These findings are underpinned by a model based on cell growth and cell cycle. Further, we numerically determine the range of validity of the scale invariance over various characteristic times of the starvation process, and find the violation of the scale invariance for slow starvation. Our results, combined with theoretical arguments, suggest the relevance of the multifork replication, which helps retaining information of cell cycle states and may thus result in the scale invariance.