Paclitaxel-Releasing Thin Biodegradable Film for Prevention of Bleb Avascularity Without Compromising Filtration in Rabbits.
Paclitaxel-Releasing Thin Biodegradable Film for Prevention of Bleb Avascularity Without Compromising Filtration in Rabbits.
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DOI：
10.1167/tvst.4.3.10
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发表时间：
2015-05
影响因子：
3
通讯作者：
Tetsuhiko Okuda;T. Higashide;M. Sakurai;Yukako Fukuhira;Hiroaki Kaneko;M. Shimomura;K. Sugiyama
中科院分区：
文献类型：
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作者：
Tetsuhiko Okuda;T. Higashide;M. Sakurai;Yukako Fukuhira;Hiroaki Kaneko;M. Shimomura;K. Sugiyama
在兔滤过手术模型中，一种蜂窝状薄膜（HPF）可预防滤过泡瘢痕形成和丝裂霉素C（MMC）相关滤过泡无血管性。在这项研究中，我们研究了是否HPF释放紫杉醇（PTX）可以防止滤过泡无血管而不影响过滤。方法对兔的一只眼进行滤过手术。将由聚（L-丙交酯-co-ε-己内酯）制成的14 μm厚HPF置于滤过部位的结膜下，蜂窝表面朝向结膜下Tenon组织。将家兔分为4组（n = 5）：1，HPF未用药组，2，HPF + PTX 50 μg组，3，HPF + 5 μg组，4，HPF + 0.5 μg组。使用超声生物显微镜定期进行眼内压（IOP）测量和滤过泡评价，持续4周，然后进行组织学检查。对第4组（实验2; n = 8）进行了更长时间的随访研究（12周）。结果在所有组中，在4周随访时，第1组中有两个滤过泡失败。PTX治疗眼的术后IOP降低明显大于第1组。第2组和第3组的滤过泡无血管区持续4周。然而，在术后4周时，在第1组和第4组中未观察到无血管区。组织学显示所有PTX组的滤过部位均存在极轻微纤维化。在实验2中，一些滤过泡在术后10周开始变得更平坦。结论：HPF释放的PTX可促进滤过泡存活，降低眼压。最低剂量的PTX（0.5 μg）可有效预防滤过泡无血管性，而不影响滤过。
PURPOSE A honeycomb-patterned film (HPF) prevents bleb scarring and mitomycin C (MMC)-related bleb avascularity in a rabbit model of filtration surgery. In this study, we examined whether a HPF-releasing paclitaxel (PTX) can prevent bleb avascularity without compromising filtration. METHODS Filtration surgery was performed in one eye of rabbits. A 14-μm thick HPF made from poly(L-lactide-co-ε-caprolactone) was placed subconjunctivally over the filtration site with the honeycomb surface turned toward the subconjunctival Tenon tissue. The rabbits were divided into four groups (n = 5 each): 1, HPF with no drug; 2, HPF + PTX 50 μg; 3, HPF + 5 μg; 4, HPF + 0.5 μg. Intraocular pressure (IOP) measurements and bleb evaluations using ultrasound biomicroscopy were performed periodically for 4 weeks followed by histological examination. A longer follow-up study (12 weeks) was performed for group 4 (experiment 2; n = 8). RESULTS Among all groups at the 4-week follow up, two blebs failed in group 1. The postoperative IOP decrease was significantly greater in PTX-treated eyes than in group 1. The bleb avascular area persisted for 4 weeks in groups 2 and 3. However, no avascular area was observed in groups 1 and 4 at 4 weeks postoperatively. Histology showed minimal fibrosis at the filtration site in all the PTX groups. In experiment 2, some blebs became flatter starting at 10 weeks after surgery. CONCLUSIONS PTX released from HPF promoted bleb survival and IOP decrease. The lowest dose of PTX (0.5 μg) was effective at preventing bleb avascularity without compromising filtration.