Fascin Expression Predicts Survival After Potentially Curative Resection of Node-positive Colon Cancer
Fascin Expression Predicts Survival After Potentially Curative Resection of Node-positive Colon Cancer
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DOI：
10.1097/pas.0b013e3181db36c0
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发表时间：
2010-05-01
影响因子：
5.6
通讯作者：
Clarke, Stephen
中科院分区：
文献类型：
--
作者：
Chan, Charles;Jankova, Lucy;Clarke, Stephen
筋膜蛋白是一种肌动蛋白捆绑蛋白，在包括结直肠癌在内的许多肿瘤中表达。它被认为是肿瘤细胞侵袭的中介和侵袭性表型的指标；然而，关于结直肠癌中筋膜蛋白与预后之间关系的报道很少。本研究的目的是：(a)研究在淋巴结阳性结肠癌切除后可能治愈的患者中，束蛋白在肿瘤中心部分和侵袭性前部的表达；(b)检查fastin表达评分方法；(c)研究筋膜蛋白表达与总生存率和其他临床病理特征之间的关系。通过对470例患者的存档组织进行微阵列免疫染色来评估筋膜蛋白的表达，这些患者在切除后至少随访了5年。其他临床病理资料已按照标准化方案进行前瞻性记录。总生存率分析采用Kaplan-Meier法和Cox回归。对于中心肿瘤组织和侵袭性前部，我们发现染色细胞的百分比足以衡量与存活相关的束蛋白表达，染色强度没有提供显著的额外信息。在这两个水平上，高筋膜蛋白表达与生存率降低之间存在显著的独立关联，尽管这种关联在中央区（校正风险比1.6,P < 0.001）比侵袭前区（校正风险比1.1,P = 0.044）强得多。筋膜蛋白表达预测总体生存，但不能取代其他常规收集的临床病理预测指标。
Fascin, an actin-bundling protein, is expressed in many neoplasms including colorectal cancer. It is considered to be a mediator of tumor cell invasion and an indicator of aggressive phenotype; however, there are few reports on the association between fascin and prognosis in colorectal cancer. The aims of this study were to: (a) investigate the expression of fascin in the central part of the tumor and at the invasive front in patients who had a potentially curative resection for node-positive colonic carcinoma; (b) examine the method of scoring fascin expression; and (c) investigate the association between fascin expression and overall survival and other clinicopathologic features. Fascin expression was assessed by immunostaining of microarrays from archived tissue of 470 patients who were followed for a minimum of 5 years after resection. Other clinicopathologic data had been recorded prospectively according to a standardized protocol. Analysis of overall survival was by the Kaplan-Meier method and Cox regression. For both central tumor tissue and the invasive front, it was found that the percentage of stained cells was a sufficient measure of fascin expression in relation to survival, with staining intensity providing no significant additional information. At both levels, there was a significant independent association between high fascin expression and diminished survival, although this association was much stronger in the central region (adjusted hazard ratio 1.6, P < 0.001) than at the invasive front (adjusted hazard ratio 1.1, P = 0.044). Fascin expression predicted overall survival but did not displace other routinely collected clinicopathologic predictors.