A Portable 3D Printer System for the Diagnosis and Treatment of Multidrug-Resistant Bacteria
A Portable 3D Printer System for the Diagnosis and Treatment of Multidrug-Resistant Bacteria
复制标题
DOI：
10.1016/j.chempr.2016.08.008
复制
发表时间：
2016-09
期刊：
影响因子：
23.5
通讯作者：
S. Glatzel;M. Hezwani;Philip J. Kitson;P. Gromski;Sophie Schürer;L. Cronin
中科院分区：
文献类型：
--
作者：
S. Glatzel;M. Hezwani;Philip J. Kitson;P. Gromski;Sophie Schürer;L. Cronin
多重耐药细菌是人类健康的主要威胁，但广谱抗生素正在失去效力。需要在实验室外筛选针对细菌感染的给定药物。为了满足这一需求，我们设计并构建了一种廉价且易于使用的基于3D打印机的系统，该系统可以轻松读取抗菌药物性能的定量测试。该平台通过使用3D打印创建无菌诊断设备，该设备自动填充生长培养基，然后通过喷墨技术将抗生素喷洒到培养基上。用于测试的样品可以通过流体端口引入，并且打印机热床用于孵育设备，从而允许在现场操作。结合各种已建立的抗菌性能测试的优点，这允许对特定抗生素和细菌进行比较。此外，该系统可以创建和测试用于治疗用途的几种抗生素制剂，我们通过研究仅被药物混合物杀死的病原体混合物来证明这一潜力。
Multidrug-resistant bacteria are a major threat to human health, but broad-spectrum antibiotics are losing efficacy. There is a need to screen a given drug against a bacterial infection outside of the laboratory. To address this need, we have designed and built an inexpensive and easy-to-use 3D-printer-based system that allows easily readable quantitative tests for the performance of antibacterial drugs. The platform creates a sterile diagnostic device by using 3D printing, and the device is filled automatically with growth medium, and then antibiotics are sprayed onto the medium by ink-jet technology. The sample for testing can be introduced via a fluid port, and the printer hot bed is used to incubate the device, allowing operation in the field. Combining advantages from various established tests of antibacterial performance, this allows the comparison of a specific antibiotics and bacteria. Also, this system can create and test several antibiotic formulations for therapeutic use, and we demonstrate this potential by investigating a mixture of pathogens that are only killed by a mixture of drugs.