Localization of neutralization epitopes on adenovirus fiber knob from species C
Localization of neutralization epitopes on adenovirus fiber knob from species C
复制标题
C 种腺病毒纤维旋钮上中和表位的定位。
DOI：
10.1099/jgv.0.000410
复制
发表时间：
2016-04-01
影响因子：
3.8
通讯作者：
Yu, Xianghui
中科院分区：
文献类型：
--
作者：
Lang, Shuai;Wang, Lizheng;Yu, Xianghui
虽然已经发现了腺病毒（AdV）血清2型（Ad2）和Ad5型纤维旋涡上潜在的中和表位，但很少有研究从更广泛的AdV血清型中鉴定旋涡上的中和表位。本研究通过对C种Ad1、Ad2、Ad5和Ad6的旋钮的序列和结构分析，在大肠杆菌中表达了多个三聚体嵌合的旋钮蛋白，并通过对小鼠和兔抗AdV多克隆血清和自然感染AdV的人血清的反应性分析，确定了旋钮上的中和表位位置。优势中和表位主要位于Ad1、Ad2和Ad5旋钮的n端部分，但似乎位于Ad6旋钮的c端部分，在兔和人群体中存在一定的个体差异。我们的研究增加了我们对adv的体液免疫反应的理解，并将有助于构建更理想的衣壳修饰的重组Ad5载体。
Although potential neutralization epitopes on the fiber knob of adenovirus (AdV) serotype 2 (Ad2) and Ad5 have been revealed, few studies have been carried out to identify neutralization epitopes on the knob from a broader panel of AdV serotypes. In this study, based on sequence and structural analysis of knobs from Ad1, Ad2, Ad5 and Ad6 (all from species C), several trimeric chimeric knob proteins were expressed in Escherichia coli to identify the locations of neutralization epitopes on the knobs by analysing their reactivity with mouse and rabbit polyclonal sera raised against AdVs and human sera with natural AdV infection. The dominant neutralization epitopes were located mainly in the N-terminal part of knobs from Ad1, Ad2 and Ad5, but they seemed to be located in the C-terminal part of the Ad6 knob, with some individual differences in rabbit and human populations. Our study adds to our understanding of humoral immune responses to AdVs and will facilitate the construction of more desirable capsid-modified recombinant Ad5 vectors.