Role of angiogenic factors: Coexpression of interleukin-8 and vascular endothelial growth factor in patients with head and neck squamous carcinoma
Role of angiogenic factors: Coexpression of interleukin-8 and vascular endothelial growth factor in patients with head and neck squamous carcinoma
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DOI：
10.1097/00005537-199905000-00002
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发表时间：
1999-05-01
期刊：
影响因子：
2.6
通讯作者：
Kreutzer, DL
中科院分区：
文献类型：
--
作者：
Eisma, RJ;Spiro, JD;Kreutzer, DL
目的/假设：血管生成已被用作多种癌症的预后指标，并且被认为是由血管生成因子控制的，包括细胞因子白细胞介素-8 (IL-8) 和血管内皮生长因子 (VEGF)。我们假设头颈肿瘤中 IL-8 和 VEGF 的体内共表达通过增强血管生成有助于维持肿瘤生长和转移。方法：免疫组织化学分析使用 33 名癌症患者和 6 名对照患者的标本进行 IL-8 和 VEGF 检测。我们使用酶联免疫吸附测定和放射免疫测定定量评估了来自同一患者的肿瘤组织匀浆中 IL-8 和 VEGF 的水平。采集每位患者的综合病史，并随后分析其与 IL-8 或 VEGF 水平的临床相关性。结果：发现IL-8和VEGF共定位于头颈鳞状细胞癌（HNSCCA）肿瘤细胞内，在头颈肿瘤标本中，IL-8水平（[38,152 +/- 1.8] x 10(5) pg/mg总蛋白[TP]）是对照（1,721 +/- 2,122 pg/mg TP）的22倍，VEGF的肿瘤水平(1,304 +/- 6,037 pg/mg TP) 比对照组 (317 +/- 400 pg/mg TP) 高出近四倍，白细胞介素 8 和 VEGF 水平被发现具有正相关性 (P 小于或等于 0.0001)，每毫克 TP 的皮克水平和/或 IL-8 和 VEGF 摩尔数表现出高水平的患者在临床上患有更具侵袭性的疾病表现为较高的 TNM 分期、较多的复发和较短的无病间隔（P 小于或等于 0.03） 结论：HNSCCA 中 IL-8 和 VEGF 的显着增加强调了这些血管生成因子在该疾病中的重要性。了解 IL-8 和 VEGF 等血管生成因子的作用和相互作用可能对 HNSCCA 的治疗有价值。
Objective/Hypothesis: Angiogenesis has been used as a prognostic indicator in a variety of cancers and is believed to be controlled by angiogenic factors, including the cytokines interleukin-8 (IL-8) and vascular endothelial growth factor (VEGF), We hypothesized that the in vivo coexpression of both IL-8 and VEGF in head and neck tumors contributes to perpetuating tumor growth and metastasis by enhancing angiogenesis, Methods: Immunohistochemical analysis for IL-8 and VEGF was performed using specimens from 33 cancer patients and 6 control patients. We quantitatively evaluated levels of IL-8 and VEGF in tumor tissue homogenates from those same patients using enzyme-linked immunosorbent assay and radioimmunoassay. Comprehensive histories of each patient were taken and later analyzed for clinical correlations with IL-8 or VEGF levels. Results: IL-8 and VEGF were found to be colocalized within the head and neck squamous cell carcinoma (HNSCCA) tumor cells, in the head and neck tumor specimens, IL-8 levels ([38,152 +/- 1.8] x 10(5) pg/mg total protein [TP]) were 22-fold greater than controls (1,721 +/- 2,122 pg/mg TP), The tumor levels of VEGF (1,304 +/- 6,037 pg/mg TP) were nearly fourfold higher than the controls (317 +/- 400 pg/mg TP), Interleukin-8 and VEGF levels were found to have a positive correlation (P less than or equal to .0001), Patients exhibiting high levels in picograms per milligram of TP and/or number of moles of IL-8 and VEGF were found to clinically have more aggressive disease manifested by higher TNM stage, more recurrences, and shorter disease-free intervals (P less than or equal to .03) Conclusions: Marked increase in HNSCCA of IL-8 and VEGF underscores the importance of these angiogenic factors in this disease. Understanding the roles and interplay of angiogenic factors such as IL-8 and VEGF may have value in the treatment of HNSCCA.