DEPENDENCE OF LOCALLY MEASURED CELLULAR DEFORMABILITY ON POSITION ON THE CELL, TEMPERATURE, AND CYTOCHALASIN-B
DEPENDENCE OF LOCALLY MEASURED CELLULAR DEFORMABILITY ON POSITION ON THE CELL, TEMPERATURE, AND CYTOCHALASIN-B
复制标题
DOI：
10.1073/pnas.79.17.5327
复制
发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
ELSON, EL
中科院分区：
文献类型：
--
作者：
PETERSEN, NO;MCCONNAUGHEY, WB;ELSON, EL
描述了一种通过直接测量活细胞对局部施加的力的机械阻力来探索细胞[小鼠成纤维细胞3 T3]表面-细胞骨架相互作用的方法。这些测量对整个细胞和其表面以下不同深度处的结构变化敏感。局部细胞变形性取决于温度和细胞骨架的完整性。细胞松弛素B可增加除细胞核外细胞所有区域的变形能力。
An approach to exploring cell [mouse fibroblast 3T3] surface-cytoskeleton interactions through direct measurements of the mechanical resistance of living cells to locally applied forces was described. These measurements are sensitive to variations in structure across the cell and at various depths below its surface. Local cellular deformability depends on the temperature and on the integrity of the cytoskeleton. Cytochalasin B increases the deformability of all regions of the cell except the nucleus.