Increased Circulating Th17 but Decreased CD4(+)Foxp3(+) Treg and CD19(+)CD1d(hi)CD5(+) Breg Subsets in New-Onset Graves' Disease.
Increased Circulating Th17 but Decreased CD4(+)Foxp3(+) Treg and CD19(+)CD1d(hi)CD5(+) Breg Subsets in New-Onset Graves' Disease.
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新发格雷夫斯病中循环 Th17 增加但 CD4( ) Foxp3( ) Treg 和 CD19( ) CD1d(hi)CD5( ) Breg 亚群减少
DOI：
10.1155/2017/8431838
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发表时间：
2017
影响因子：
--
通讯作者：
Teng W
中科院分区：
文献类型：
--
作者：
Qin J;Zhou J;Fan C;Zhao N;Liu Y;Wang S;Cui X;Huang M;Guan H;Li Y;Shan Z;Teng W
Th17和调节性淋巴细胞亚群如Tregs和Bregs已被报道在自身免疫性疾病中发挥重要作用。这项工作的目的是对新发Graves病(GD)患者的循环Th17、Tregs和Bregs进行定量研究。20例GD患者和20例健康对照参与本研究。用流式细胞仪检测外周血中CD4+IL-17+Th17、CD4+Foxp3+Tregs和CD19+CD1dhiCD5+Bregs的表达，同时用实时定量聚合酶链式反应检测γ-t、IL-17和IL-10的基因表达。GD组Tregs和Bregs比例及Foxp3基因表达均显著低于健康对照组，而IL-10无明显变化。GD组Th1 7细胞频率、RoR、γ、t、IL-17基因表达均显著高于对照组。此外，GD组Th17/Treg比值也明显高于对照组。Th17与TSAb呈显著正相关(r=0.656，p<；0.001)，Treg/Breg与TSAb呈显著负相关(r=−0.339，p=0.032；r=−0.759，p<；0.001)。这项研究表明，Th17增强和Treg反应受损，以及CD19+CD1dhiCD5+Breg细胞数量减少，参与了GD的发病。
Th17 and regulatory lymphocyte subsets such as Tregs and Bregs have been reported to play important roles in autoimmune diseases. The aim of this work was to perform quantitative studies of circulating Th17, Tregs, and Bregs in patients with new-onset Graves' disease (GD). Twenty GD patients and 20 healthy controls were involved in this study. Blood samples were taken for flow cytometry detection of CD4+IL-17+ Th17, CD4+Foxp3+ Tregs, and CD19+CD1dhiCD5+ Bregs and meanwhile, for real-time PCR measurement of gene expressions of RORγt, IL-17 and IL-10. The proportions of Tregs and Bregs as well as the Foxp3 gene expression but not IL-10 were significantly decreased in GD group compared with the healthy controls. The frequency of Th17 together with the gene expressions of RORγt and IL-17 were significantly increased in the GD group. Furthermore, the Th17/Treg ratio was also significantly higher in GD group. A significant positive correlation between Th17 and TSAb (r = 0.656, p < 0.001) but significant negative correlations between Treg/Breg and TSAb (r = −0.339, p = 0.032; r = −0.759, p < 0.001) were identified among the participants. This study indicated that increased Th17 and impaired Treg responses, along with a decreased number of CD19+CD1dhiCD5+ Breg cells, were involved in GD pathogenesis.
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影响因子：
5.4
作者：
Shi, L.;Bi, M.;Teng, W.
通讯作者：
Teng, W.
影响因子：
6
作者：
Yanaba, Koichi;Yoshizaki, Ayumi;Sato, Shinichi
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Sato, Shinichi
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6
作者：
Yang, Min;Deng, Jun;Lu, Liwei
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影响因子：
4.6
作者：
Cui, Donghua;Zhang, Lili;Shen, Bo
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Shen, Bo
影响因子：
3.2
作者：
Mitra, A.;Raychaudhuri, S. K.;Raychaudhuri, S. P.
通讯作者：
Raychaudhuri, S. P.