Control of Blood Glucose Levels in Alloxan-diabetic Rabbits by Iontophoresis of Insulin
Control of Blood Glucose Levels in Alloxan-diabetic Rabbits by Iontophoresis of Insulin
复制标题
胰岛素离子电渗疗法控制四氧嘧啶糖尿病兔的血糖水平
作者：
B. Kári
采用阴极离子导入法给23只四氧嘧啶糖尿病雄性新西兰白色家兔输注胰岛素。使用0.2-0.8 mA的电流从含有10-500 U/ml胰岛素浓度的水溶液的储液器输送胰岛素。无论使用的电流水平如何，在开启电流的1小时内，血糖水平降低，血清胰岛素浓度升高。此外，在大多数情况下，在电流关闭后，血糖水平继续降低，血清胰岛素浓度继续增加，这表明离子电渗可以用于在皮肤和皮下组织中积累胰岛素。通过离子电渗法输送的胰岛素量可以通过使用高达0.4 mA的电流水平来控制;将电流增加到0.8 mA不会输送更多的胰岛素。这可能是由于在0.8 mA时产生的氢氧根离子更多，与胰岛素竞争携带电流，从而减缓胰岛素的运动。输送的胰岛素的量也可以通过可用于离子电渗的胰岛素的量来控制，即，当胰岛素储库浓度增加时，在相同的电流水平下输送更多的胰岛素。最后，皮肤准备在控制胰岛素输送方面也很重要。为了输送足够的胰岛素以降低血糖水平，角质层必须通过轻轻刮擦来破坏或去除。
Cathodal iontophoresis was used to deliver insulin in 23 alloxan-diabetic, male, New Zealand white rabbits. Currents of 0.2–0.8 mA were used to deliver insulin from reservoirs containing insulin concentrations of 10–500 U/ml in aqueous solution. Regardless of the level of current used, within 1 h of turning the current on, blood glucose levels decreased and serum insulin concentrations increased. Moreover, in most cases, blood glucose levels continued to decrease and serum insulin concentrations continued to increase after the current was turned off, suggesting that iontophoresis could be used to accumulate insulin in the skin and subcutaneous tissues. The amount of insulin that was delivered by iontophoresis could be controlled by the level of current used up to 0.4 mA; increasing the current to 0.8 mA did not deliver more insulin. This may have been due to greater production of hydroxide ions at 0.8 mA, which competed with insulin to carry the current, thus slowing the movement of insulin. The amount of insulin delivered could also be controlled by the amount of insulin available for iontophoresis, i.e., as the insulin reservoir concentration increased, more insulin was delivered at the same current level. Finally, skin preparation was also important in controlling insulin delivery. To deliver enough insulin to reduce blood glucose levels, the stratum corneum had to be disrupted or removed by gentle scraping.