Tensiometric and Phase Domain Behavior of Lung Surfactant on Mucus-like Viscoelastic Hydrogels.
Tensiometric and Phase Domain Behavior of Lung Surfactant on Mucus-like Viscoelastic Hydrogels.
复制标题
肺表面活性剂在粘液状粘弹性水凝胶上的张力和相域行为。
DOI：
10.1021/acsami.6b00294
复制
发表时间：
2016
影响因子：
9.5
通讯作者：
Fiegel,Jennifer
中科院分区：
文献类型：
--
作者：
Schenck,DanielM;Fiegel,Jennifer
肺表面活性物质已被观察到在气道衬里流体的所有表面，并且是正常肺功能的重要贡献者。在传导气道中，表面活性剂膜位于粘弹性粘液凝胶的顶部。在这项工作中，我们报告肺表面活性物质在粘液样粘弹性凝胶的气-液界面的张力和相域行为的表征。聚（丙烯酸）水凝胶配制成作为一个模型粘液与散装流变学特性相匹配的气管支气管粘液分泌物。将Infasurf（Calfactant）（一种源自小牛肺提取物的市售肺表面活性剂）涂布到水凝胶表面上。对Infasurf薄膜在水凝胶上的表面张力降低能力和松弛进行了定量分析，并与Infasurf在含水亚相上的行为进行了比较。在表面压缩过程中的Infasurf相域，其特征在于通过荧光显微镜和相移干涉。我们观察到，增加模型粘液水凝胶的体积粘弹性能降低了Infasurf薄膜降低表面张力和抑制薄膜松弛的能力。在形成Infasurf凝聚相域从较小的，更球形的域大，团聚的，多层结构的转移，观察到的亚相的粘弹性增加。这些研究表明，肺表面活性剂在粘弹性表面上的表面行为，如在传导气道中发现的那些，与含水的、负载表面活性剂的系统显著不同。
Lung surfactant has been observed at all surfaces of the airway lining fluids and is an important contributor to normal lung function. In the conducting airways, the surfactant film lies atop a viscoelastic mucus gel. In this work, we report on the characterization of the tensiometric and phase domain behavior of lung surfactant at the air–liquid interface of mucus-like viscoelastic gels. Poly(acrylic acid) hydrogels were formulated to serve as a model mucus with bulk rheological properties that matched those of tracheobronchial mucus secretions. Infasurf (Calfactant), a commercially available pulmonary surfactant derived from calf lung extract, was spread onto the hydrogel surface. The surface tension lowering ability and relaxation of Infasurf films on the hydrogels was quantified and compared to Infasurf behavior on an aqueous subphase. Infasurf phase domains during surface compression were characterized by fluorescence microscopy and phase shifting interferometry. We observed that increasing the bulk viscoelastic properties of the model mucus hydrogels reduced the ability of Infasurf films to lower surface tension and inhibited film relaxation. A shift in the formation of Infasurf condensed phase domains from smaller, more spherical domains to large, agglomerated, multilayer structures was observed with increasing viscoelastic properties of the subphase. These studies demonstrate that the surface behavior of lung surfactant on viscoelastic surfaces, such as those found in the conducting airways, differs significantly from aqueous, surfactant-laden systems.