Changes in the expression levels of tight junction components during reconstruction of tight junction from mucosal lesion by intestinal ischemia/reperfusion
Changes in the expression levels of tight junction components during reconstruction of tight junction from mucosal lesion by intestinal ischemia/reperfusion
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DOI：
10.1007/s13318-013-0151-z
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发表时间：
2013-09
影响因子：
1.9
通讯作者：
Y. Takizawa;Hisanao Kishimoto;M. Tomita;M. Hayashi
中科院分区：
文献类型：
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作者：
Y. Takizawa;Hisanao Kishimoto;M. Tomita;M. Hayashi
在上皮细胞中，紧密连接（Tight junction， TJ）是由细胞间连接复合体的最顶端组分组成的；TJ具有细胞极性，是上皮屏障功能的主要决定因素。在本研究中，为了明确TJ重建和功能获得所需的成分，我们检测了肠I/R对小肠黏膜病变的依赖，即claudin家族和支架蛋白mRNA和蛋白表达的变化。我们采用弹簧秤和手术缝合线制作的活体肠道I/R模型，分别采用实时RT-PCR和Western blotting检测TJ组分mRNA和蛋白的表达水平。观察肠I/R对TJ组分mRNA和蛋白表达水平的影响。其中，claudin-2和claudin-4发生了特征性变化。此外，多pdz结构域蛋白（MPDP） mRNA的表达行为与claudin-4相似。综上所述，在肠I/R粘膜病变TJ的恢复过程中，提示claudin-2和claudin-4分别强烈参与TJ的重建和功能获得。此外，支架蛋白MPDZ在这些过程中也起着重要作用。
Tight junction (TJ) is composed of the most apical components of the intercellular junctional complex in epithelial cells; TJ has cell polarity and functions as a major determinant of epithelial barrier function. In this study, to clarify the components of TJ required for its reconstruction and functional acquisition, we examined the changes in intestinal mucosal structure that depended on mucosal lesion by intestinal I/R, that is, the changes in mRNA and protein expression of the claudin family and scaffold proteins. We used an in vivo intestinal I/R model made using the spring scale and surgical sutures, and examined the mRNA and protein expression levels of TJ components by real-time RT-PCR and Western blotting, respectively. Changes in mRNA and protein expression levels of TJ components by intestinal I/R were observed. Among them, characteristic changes were observed in claudin-2 and claudin-4. In addition, the expression behavior of multi-PDZ domain protein (MPDP) mRNA was similar to that of claudin-4. In conclusion, in the recovery process of TJ from mucosal lesion by intestinal I/R, it was suggested that claudin-2 and claudin-4 strongly participate in the reconstruction and functional acquisition of TJ, respectively. Furthermore, it was suggested that MPDZ, which is scaffold protein, also has an important role in these processes.