The effect of surface microtopography of poly(dimethylsiloxane) on protein adsorption, platelet and cell adhesion
The effect of surface microtopography of poly(dimethylsiloxane) on protein adsorption, platelet and cell adhesion
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聚二甲基硅氧烷表面微形貌对蛋白质吸附、血小板和细胞粘附的影响
DOI：
10.1016/j.colsurfb.2009.02.018
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发表时间：
2009-07-01
影响因子：
5.8
通讯作者：
Yang, Bai
中科院分区：
文献类型：
--
作者：
Chen, Hong;Song, Wei;Yang, Bai
利用化学均相聚二甲基硅氧烷(PDMS)表面点状突起结构，研究了表面微形貌对蛋白质吸附和后续生物响应的影响。纤维蛋白原(Fg)和纤维连接蛋白(Fn)分别因其对血小板和细胞黏附的影响而被选为模型蛋白。用~(125)I标记的FG和异硫氰酸荧光素(FITC)研究了它在平面和图案化表面的吸附。与平坦表面相比，花纹表面对FG的吸附增加了46%。然而，图案化表面的表面积仅比平坦表面的表面积大8%。因此，比表面积的增加并不是蛋白质吸附增加的唯一因素。在图案化表面可见清晰的荧光图案，表明吸附的FG呈规则分布，并主要吸附在突起的侧面和山谷。这种FG的分布和局部浓缩可能是富血小板血浆(PRP)和流动全血(FWB)附着在图案表面的特殊位置。此外，表面形貌和预吸附FN的不同组合也会影响L929细胞的粘附性。预吸附FN的平坦表面是L929细胞扩散的最佳衬底，而原始花纹表面是较差的衬底。(C)2009爱思唯尔B.V.保留所有权利。
Chemical homogeneous poly(dimethylsiloxane) (PDMS) surface with dot-like protrusion pattern was used to investigate the individual effect of surface microtopography on protein adsorption and subsequent biological responses. Fibrinogen (Fg) and fibronectin (Fn) were chosen as model proteins due to their effect on platelet and cell adhesion, respectively. Fg labeled with (125)I and fluorescein isothiocyanate (FITC) was used to study its adsorption on flat and patterned surfaces. Patterned surface has a 46% increase in the adsorption of Fg when compared with flat surface. However, the surface area of the patterned surface was only 8% larger than that of the flat surface. Therefore, the increase in the surface area was not the only factor responsible for the increase in protein adsorption. Clear fluorescent pattern was visualized on patterned surface, indicating that adsorbed Fg regularly distributed and adsorbed most on the flanks and valleys of the protrusions. Such distribution and local enrichment of Fg presumably caused the specific location of platelets adhered from platelet-rich plasma (PRP) and flowing whole blood (FWB) on patterned surface. Furthermore, the different combination of surface topography and pre-adsorbed Fn could influence the adhesion of L929 cells. The flat surface with pre-adsorbed Fn was the optimum substrate while the virgin patterned surface was the poor substrate in terms of L929 cells spread. (C) 2009 Elsevier B.V. All rights reserved.