PHASE FLUOROMETRIC STERILIZABLE OPTICAL OXYGEN SENSOR
PHASE FLUOROMETRIC STERILIZABLE OPTICAL OXYGEN SENSOR
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DOI：
10.1002/bit.260431119
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发表时间：
1994-05-01
影响因子：
3.8
通讯作者：
RAO, G
中科院分区：
文献类型：
--
作者：
BAMBOT, SB;HOLAVANAHALI, R;RAO, G
我们在这里报告的低成本，光学氧传感器作为一个有吸引力的替代广泛使用的安培克拉克型氧电极测量溶解氧的紧张局势在细胞培养和生物反应器。我们的传感器是基于差分猝灭的荧光寿命的发色团在响应氧分压。这被测量为当被强度调制光束激发时由于氧猝灭而导致的来自发色团的荧光发射的相移。在这篇文章中，我们展示了基于寿命的光学方法的优势，基于强度的光学方法和安培电极。我们的传感器特别适用于测量生物反应器中的溶解氧。它是可高压灭菌的，不需要维护，并且解决了长期稳定性、校准漂移和在密集微生物培养物中可靠测量低氧张力的问题，这些问题限制了Clark型电极的应用。(C)John Wiley & Sons，Inc.
We report here on a low-cost, optical oxygen sensor as an attractive alternative to the widely used amperometric Clark-type oxygen electrode for measuring dissolved oxygen tensions in cell cultures and bioreactors. Our sensor is based on the differential quenching of the fluorescence lifetime of a chromophore in response to the partial pressure of oxygen. This is measured as a phase shift in fluorescence emission from the chromophore due to oxygen quenching when excited by an intensity modulated beam of light. In this article we demonstrate the advantages of lifetime-based optical methods over both intensity based optical methods and amperometric electrodes. Our sensor is particularly suitable for measuring dissolved oxygen in bioreactors. it is autoclavable, is free of maintenance requirements, and solves the problems of long-term stability, calibration drifts, and reliable measurement of low oxygen tensions in dense microbial cultures that limit the utility of Clark-type electrodes. (C) 1994 John Wiley & Sons, Inc.