[Mechanism of lung injury of rats induced by inhalation of white smoke from burning smoke pot].
[Mechanism of lung injury of rats induced by inhalation of white smoke from burning smoke pot].
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DOI：
10.3760/cma.j.issn.1009-2587.2018.07.010
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发表时间：
2018-07-20
影响因子：
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通讯作者：
Tong, Y L
中科院分区：
文献类型：
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作者：
Cui, P;Xin, H M;Tong, Y L
目的：探讨燃烧烟壶产生的白色烟雾对大鼠肺损伤的机制。方法：48只SD道利大鼠按随机数字表法分为对照组（n=12）和损伤组（n=36）。致伤组大鼠置于充满燃烧烟壶产生的白色烟雾的烟雾致伤实验装置中5 min，造成肺损伤;对照组大鼠置于充满空气的烟雾致伤实验装置中5 min，造成假损伤。分别于伤后6、24、72小时取伤组6只和伤后72小时取对照组6只，HE染色观察两组大鼠肺组织病理学改变和病理学评分，检测核因子-κ B的表达采用逆转录聚合酶链反应（RT-PCR）检测大鼠肺组织中NF-κ B p65 mRNA的表达，并检测肿瘤坏死因子α（TNF α）的含量通过酶联免疫吸附试验测定大鼠肺组织中的肿瘤坏死因子-α（TNF-α）、白细胞介素1 β（IL-1 β）和IL-6。数据处理采用单因素方差分析和t检验。结果：PIH 72时，对照组大鼠肺组织结构清晰完整，无炎性细胞浸润。PIH 6时损伤组大鼠肺组织水肿、出血、炎性细胞浸润。PIH 24时损伤组大鼠肺组织水肿、出血、炎性细胞浸润加重。妊高征72天时，损伤组大鼠肺组织水肿面积扩大，出血明显，炎性细胞浸润。PIH 6、24、72时损伤组大鼠肺组织病理评分分别为（3.43±0.86）、（5.39±0.93）、（9.99±0.84）分，明显高于PIH 72时对照组大鼠（2.11±0.20）分[t=3.659，8.450，22.355，P
Objective: To explore mechanism of lung injury of rats induced by inhalation of white smoke from burning smoke pot. Methods: Forty-eight Sprague Dawley rats were divided into control group (n=12) and injury group (n=36) according to the random number table. Rats in injury group were placed in smoke-induced injury experimental equipment fulled with white smoke from burning smoke pot for 5 minutes to make lung injury, and rats in control group were placed in smoke-induced injury experimental equipment fulled with air for 5 minutes to make sham injury. Six rats in injury group at post injury hour (PIH) 6, 24, and 72 and six rats in control group at PIH 72 were collected to observe pathological changes of lung tissue and pathological score of rats in the two groups by hematoxylin-eosin staining, to detect expression of nuclear factor-kappaB (NF-kappaB) p65 mRNA in lung tissue of rats by reverse transcriptional polymerase chain reaction, and to detect content of tumor necrosis factor alpha (TNF-alpha), interleukin 1beta (IL-1beta), and IL-6 in lung tissue of rats by enzyme-linked immunosorbent assay. Data were processed with one-way analysis of variance and t test. Results: At PIH 72, lung tissue structure of rats in control group was clear and complete, with no inflammatory cell infiltration. At PIH 6, there was edema, hemorrhage, and inflammatory cell infiltration in lung tissue of rats in injury group. At PIH 24, edema, hemorrhage, and inflammatory cell infiltration in lung tissue of rats in injury group aggravated. At PIH 72, area of edema in lung tissue of rats in injury group was enlarged, with obvious hemorrhage and inflammatory cell infiltration. At PIH 6, 24, and 72, pathological score of lung tissue of rats in injury group was (3.43±0.86), (5.39±0.93), and (9.99±0.84) points, respectively, obviously higher than that of rats in control group at PIH 72 [(2.11±0.20) points, t=3.659, 8.450, 22.355, P