Two control regions for eukaryotic tRNA gene transcription.
Two control regions for eukaryotic tRNA gene transcription.
复制标题
真核 tRNA 基因转录的两个控制区域。
DOI：
10.1073/pnas.77.6.3365
复制
发表时间：
1980
影响因子：
11.1
通讯作者：
D. Söll
中科院分区：
文献类型：
--
作者：
D. DeFranco;O. Schmidt;D. Söll
研究表明，两个具有相同编码序列的果蝇 tRNALys 基因在非洲爪蟾卵母细胞核提取物中的转录效率截然不同。使用重组质粒（其中这些基因的 5' 侧翼序列被“转换”或被定义的 pBR322 序列替换）揭示了 tRNA 基因转录的两个控制区。包含成熟tRNA编码序列（以及可能其3'-侧翼序列）的内部控制区足以启动转录，并且包含5'-侧翼序列的外部控制区抑制该转录。所有转录本都有短前导序列。从截短的 tRNALys 基因（受体茎中缺少单个碱基对）转录的改变的前体 tRNA 在体外不能很好地加工。
Two Drosophila tRNALys genes with identical coding sequences were shown to transcribe with very different efficiences in nuclear extracts from Xenopus oocytes. The use of recombinant plasmids in which the 5'-flanking sequences of these genes were either "switched" or replaced by defined pBR322 sequences revealed two control regions for tRNA gene transcription. An internal control region comprising the mature tRNA coding sequence (and possibly its 3'-flanking sequences) is sufficient for transcription initiation, and an external control region comprising the 5'-flanking sequences represses this transcription. All transcripts have short leader sequences. Altered precursor tRNAs transcribed from truncated tRNALys genes (missing a single base pair in the acceptor stem) are not processed well in vitro.