Icariin regulates the proliferation and apoptosis of human ovarian cancer cells through microRNA-21 by targeting PTEN, RECK and Bcl-2
Icariin regulates the proliferation and apoptosis of human ovarian cancer cells through microRNA-21 by targeting PTEN, RECK and Bcl-2
复制标题
DOI：
10.3892/or.2015.3891
复制
发表时间：
2015-06-01
期刊：
影响因子：
4.2
通讯作者：
Zhao, Fujie
中科院分区：
文献类型：
--
作者：
Li, Jingwei;Jiang, Kailei;Zhao, Fujie
淫羊藿苷是在传统中药植物淫羊藿中发现的主要活性成分，并且表现出多种药理作用，例如增强免疫功能、抗癌活性、改善心血管功能和调节内分泌。然而，淫羊藿苷对卵巢癌的作用及其相关机制尚未见报道。在本研究中，我们的目的是验证淫羊藿苷是否抑制人卵巢癌细胞的增殖和增加凋亡，其分子机制，以建立一个协会，并确定潜在的治疗靶点。本研究以不同浓度的淫羊藿苷处理卵巢癌A2780细胞，采用MTT法检测细胞活力。流式细胞术和caspase-3比色法检测A2780细胞凋亡的变化。使用qPCR分析来分析A2780细胞中的miR-21表达。Western blot分析PTEN、RECK和Bcl-2蛋白表达。转染microRNA-21（miR-21）和anti-miR-21，观察其与细胞增殖和凋亡相关的靶基因的表达。淫羊藿苷同时抑制A2780细胞增殖，加速凋亡，增加caspase-3活性。在卵巢癌A2780细胞中，淫羊藿苷显著降低miR-21的表达水平，增加PTEN和RECK蛋白的表达水平，降低Bcl-2蛋白的表达水平。值得注意的是，miR-21通过靶向卵巢癌A2780细胞中的PTEN、RECK和Bcl-2来调节淫羊藿苷对细胞增殖和凋亡的潜在抗癌作用。我们的研究结果表明，淫羊藿苷是一个很好的候选抗肿瘤药物，表现出对卵巢癌细胞的抗癌疗效。miR-21及其靶基因可能在淫羊藿苷抗肿瘤作用的分子机制中发挥重要作用。
Icariin is the main active ingredient found in the traditional Chinese medicinal plant Epimedium, and exhibits various pharmacological effects such as enhanced immune function, anticancer activity, improved cardiovascular function and endocrine adjustment. However, the effect of icariin on ovarian cancer and the related mechanism have never been investigated. In the present study, we aimed to verify whether icariin inhibits the proliferation and increases the apoptosis of-human ovarian cancer cells, and its molecular mechanism in order to establish an association and identify potential therapeutic targets. In the present study, ovarian cancer A2780 cells were treated with various concentrations of icariin, and the cell viability was evaluated by 3,3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Flow cytometry and caspase-3 colorimetric assay were performed to observe apoptotic changes in the A2780 cells. qPCR analysis was used to analyze miR-21 expression in the A2780 cells. Western blot analysis was used to assess PTEN, RECK and Bcl-2 protein expression. Transfection of microRNA-21 (miR-21) and anti-miR-21 was used to investigate expression of its target genes associated with cell proliferation and apoptosis. Icariin concomitantly suppressed cell proliferation, accelerated apoptosis and increased caspase-3 activity in the A2780 cells. In the ovarian cancer A2780 cells, icariin substantially decreased the miR-21 expression level, increased PTEN and RECK protein expression levels and decreased the Bcl-2 protein expression level. Notably, miR-21 regulated the potential anticancer effects of icariin on cell proliferation and apoptosis by targeting PTEN, RECK and Bcl-2 in the ovarian cancer A2780 cells. Our results demonstrated that icariin is an excellent candidate antitumor agent which exhibits an anticancer curative effect on ovarian cancer cells. miR-21 and its target genes may play a vital role in the molecular mechanism of the anticancer effects of icariin.