Effects of Cryopreservation on Sperm with Cryodiluent in Viviparous Black Rockfish (Sebastes schlegelii).
Effects of Cryopreservation on Sperm with Cryodiluent in Viviparous Black Rockfish (Sebastes schlegelii).
复制标题
冷冻稀释液对卵生黑岩鱼精子冷冻保存的影响。
DOI：
10.3390/ijms23063392
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发表时间：
2022-03-21
影响因子：
5.6
通讯作者：
Qi J
中科院分区：
文献类型：
--
作者：
Niu J;Wang X;Liu P;Liu H;Li R;Li Z;He Y;Qi J
黑石鱼是东亚地区一种重要的经济鱼类。关于黑岩鱼精子的冷冻保存，鲜有人提及。本研究通过对精子各项参数的检测，从48种冷冻稀释液中筛选出最佳冷冻稀释液。转录组和甲基组分析进一步探讨了不可避免的低温损伤的分子机制。结果表明，即使在最佳冷冻稀释液(FBS+15%Gly)的作用下，冷冻保存对精子的存活率、DNA完整性、线粒体活性、总ATPase和LDH也有负面影响。转录组和甲基组分析表明，冷冻对179个基因的表达和1266个基因的甲基化有影响。这些基因在GO死亡途径、G蛋白偶联受体信号通路、对外界刺激的反应和磷脂酶D信号通路的KEGG通路以及异源和碳水化合物代谢通路中都得到了丰富。PIK3CA和CcNA2在蛋白质-蛋白质相互作用网络中的作用被突出，并在转录组和甲基组之间的7个重叠基因中鉴定出与精子质量相关的印记基因Mest。总之，优化了黑鱼精子的冷冻稀释液，为冷冻保存提供了一种可行的方法。转录组和甲基组数据进一步揭示了冷冻损伤的潜在分子机制，为改进黑鱼的冷冻保存方法提供了线索。
Black rockfish is an economically important fish in East Asia. Little mention has been paid to the sperm cryopreservation in black rockfish. In this study, the optimal cryodiluent was selected from 48 combinations by detecting various sperm parameters. Transcriptome and methylome analysis were further performed to explore the molecular mechanism of inevitable cryoinjuries. The results showed that cryopreservation had negative effects on the viability, DNA integrity, mitochondrial activity, total ATPase and LDH of sperm even with optimal cryodiluent (FBS + 15% Gly). Transcriptome and methylome analysis revealed that the expression of 179 genes and methylation of 1266 genes were affected by cryopreservation. These genes were enriched in GO terms of death, G-protein coupled receptor signaling pathway, response to external stimulus and KEGG pathways of phospholipase D signaling pathway and xenobiotic and carbohydrate metabolism pathways. The role of PIK3CA and CCNA2 were highlighted in the protein-protein interaction network, and the sperm quality-related imprinted gene mest was identified among the 7 overlapping genes between transcriptome and methylome. Overall, the cryodiluent for black rockfish sperm was optimized, providing a feasible method for cryopreservation. The transcriptome and methylome data further demonstrated the underlying molecular mechanisms of cryoinjuries, proving clues for improvement of cryopreservation method of black rockfish.
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影响因子：
6.1
作者：
Khambata, Kushaan;Raut, Sanketa;Balasinor, Nafisa H.
通讯作者：
Balasinor, Nafisa H.
影响因子：
2
作者：
Figueroa, E.;Valdebenito, I.;Farias, J. G.
通讯作者：
Farias, J. G.
影响因子：
2.5
作者：
Depince, Alexandra;Gabory, Anne;Labbe, Catherine
通讯作者：
Labbe, Catherine
Soapnuke：MAPREDUCE加速支持的软件，用于集成质量控制和预处理高通量测序数据。
影响因子：
9.2
作者：
Chen Y;Chen Y;Shi C;Huang Z;Zhang Y;Li S;Li Y;Ye J;Yu C;Li Z;Zhang X;Wang J;Yang H;Fang L;Chen Q
通讯作者：
Chen Q
影响因子：
4.1
作者：
Breitbart, Haim;Rotman, Tali;Etkovitz, Nir
通讯作者：
Etkovitz, Nir