Simultaneous determination of uric acid and ascorbic acid at a ferrocenium-thioglycollate modified electrode
Simultaneous determination of uric acid and ascorbic acid at a ferrocenium-thioglycollate modified electrode
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DOI：
10.1007/s00216-006-0873-8
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发表时间：
2006-12-01
影响因子：
4.3
通讯作者：
Tao, Haisheng
中科院分区：
文献类型：
--
作者：
Fang, Bin;Jiao, Shoufeng;Tao, Haisheng
巯基乙酸盐在金电极表面的自组装膜（SAMS）被用作二茂铁离子静电吸附的基底界面。电化学阻抗谱（EIS）和循环伏安法（CV）的电化学性能进行了评价的超分子膜。在磷酸盐缓冲溶液（PBS，pH 7.0）中，裸金电极不能区分抗坏血酸（AA）和尿酸（UA）的氧化峰，而巯基乙酸铁修饰电极能有效地分离它们。在差示脉冲伏安法中，所制备的金电极能分离AA和UA信号，实现AA和UA的同时测定。在最佳条件下，在1.0 × 10（-6）~ 5.0 × 10（-4）M范围内，AA和UA的检出限分别为2.0 × 10（-7）和1.0 × 10（-7）M。通过对人尿中AA和UA的测定，证明了所制备的电极的适用性。
Self-assembled monolayers (SAMS) of chemisorbed thioglycollate on a gold electrode surface have been used as a base interface for the electrostatic adsorption of ferrocenium ion. Electrochemical impedance spectra (EIS) and cyclic voltammetry (CV) were used to evaluate the electrochemical properties of the supramolecular film. The bare gold electrode failed to distinguish the oxidation peaks of ascorbic acid (AA) and uric acid (UA) in phosphate buffer solution (PBS, pH 7.0), while the ferricinium-thioglycollate modified electrode could separate them efficiently. In differiential pulse voltammetric measurements, the prepared gold electrode could separate AA and UA signals, allowing the simultaneous determination of AA and UA. Under optimal conditions and within the linear range of 1.0x10(-6) to 5.0x10(-4) M, the detection limits of AA and UA achieved were 2.0x10(-7)supercript stop and 1.0x10(-7) M, respectively. The applicability of the prepared electrode was demonstrated by measuring AA and UA in human urine without any pretreatment.