Radiation-Induced Delayed Bystander-Type Effects Mediated by Hemopoietic Cells
Radiation-Induced Delayed Bystander-Type Effects Mediated by Hemopoietic Cells
复制标题
DOI：
10.1667/rr1937.1
复制
发表时间：
2010-06-01
影响因子：
3.4
通讯作者：
Wright, Eric G.
中科院分区：
文献类型：
--
作者：
Burr, Karen L.;Robinson, Joanne T.;Wright, Eric G.
与电离辐射照射有关的遗传损伤和细胞死亡一般归因于能量沉积在细胞核中引起的DNA损伤。然而，辐射诱导的旁观者效应的报告，其中DNA损伤是产生在未照射的细胞作为一个结果与照射细胞的通信，表明其他机制。目前，大部分信息都是使用体外系统获得的，旁观者因子的体内意义尚不清楚。在这项研究中，我们表明，在体内产生的信号在CBA/Ca小鼠骨髓中照射前24小时，但不是立即postrradiation 4戈伊的γ射线，能够诱导DNA损伤和非辐射骨髓细胞凋亡。信号传导机制涉及FasL、TNF-α、一氧化氮和超氧化物，巨噬细胞被认为是损伤信号的来源。这种延迟的旁观者型损伤表明，在考虑辐射照射后果的机制时，研究辐射照射后的组织反应以及照射时的影响非常重要。2010年，辐射研究学会
Genetic lesions and cell death associated with exposure to ionizing radiation have generally been attributed to DNA damage arising as a consequence of deposition of energy in the cell nucleus. However, reports of radiation-induced bystander effects, in which DNA damage is produced in nonirradiated cells as a consequence of communication with irradiated cells, indicate additional mechanisms. At present, most information has been obtained using in vitro systems, and the in vivo significance of bystander factors is not clear. In this study we show that signals generated in vivo in the bone marrow of CBA/Ca mice irradiated with 4 Gy gamma rays 24 h previously, but not immediately postirradiation, are able to induce DNA damage and apoptosis in nonirradiated bone marrow cells. The signaling mechanism involves FasL, TNF-alpha, nitric oxide and superoxide and macrophages are implicated as a source of damaging signals. Such delayed bystander-type damage demonstrates the importance of studying tissue responses subsequent to the radiation exposure as well as effects at the time of irradiation when considering the mechanisms underlying the consequences of radiation exposures. C 2010 by Radiation Research Society