Tescalcin, a novel gene encoding a putative EF-hand Ca2+-binding protein, Col9a3, and renin are expressed in the mouse testis during the early stages of gonadal differentiation
Tescalcin, a novel gene encoding a putative EF-hand Ca2+-binding protein, Col9a3, and renin are expressed in the mouse testis during the early stages of gonadal differentiation
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DOI：
10.1210/en.142.1.455
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发表时间：
2001-01-01
期刊：
影响因子：
4.8
通讯作者：
Berkovitz, GD
中科院分区：
文献类型：
--
作者：
Perera, EM;Martin, H;Berkovitz, GD
为了鉴定在发育中的睾丸中差异表达的基因，我们使用了小鼠胚胎在交配后13.5天（dpc）的性腺互补DNA的代表性差异分析。鉴定了三个基因。其中之一是一个新的基因被称为testalcin，编码一个假定的EF手钙结合蛋白。开放阅读框由642个核苷酸组成，编码214个氨基酸。预测的氨基酸序列的分析揭示了N-豆蔻酰化基序和几个磷酸化位点，除了EF-手Ca2+结合域。睾丸素信使RNA（mRNA）在胎儿睾丸中有表达，而在卵巢和中肾中无表达，仅限于睾丸索。它的表达首先在雄性性腺中检测到在11.5 dpc和表现出的模式与在睾丸分化的早期阶段在睾丸中的作用一致。睾丸素在Kit（W/W-v）小鼠的睾丸中表达，表明其不依赖于生殖细胞的存在。另外两个基因是胶原IX α 3（Col9a3）和肾素。在11.5 dpc时，雄性和雌性性腺中存在低水平的Col9a3表达。此后，它在雄性中显著上调，但在雌性中保持非常低。Col9a3的表达仅限于睾丸索，并且在Kit（W/W-v）小鼠的睾丸中也检测到。12.5dpc时，在睾丸中首次检测到肾素mRNA，此后逐渐增加，16.5dpc时达到高峰。肾素mRNA定位于生殖腺和中肾交界处的细胞中。使用标准条件未检测到卵巢中的肾素表达。
To identify genes that are differentially expressed in the developing testis we used representational difference analysis of complementary DNA from gonads of mouse embryos at 13.5 days postcoitum (dpc). Three genes were identified. One of them was a novel gene termed tescalcin that encoded a putative EF-hand Ca2+-binding protein. The open reading frame consisted of 642 nucleotides encoding a protein with 214 amino acids. Analysis of the predicted amino acid sequence revealed an N-myristoylation motif and several phosphorylation sites in addition to an EF-hand Ca2+-binding domain. Tescalcin messenger RNA (mRNA) was present in fetal testis, but not in ovary or mesonephros, and was restricted to the testicular cords. Its expression was first detected in the male gonad at 11.5 dpc and demonstrated a pattern consistent with a role in the testis at the early stages of testis differentiation. Tescalcin is expressed in the testis of Kit(W/W-v) mice, indicating that it is not dependent on the presence of germ cells. The other two genes identified were collagen IX alpha3 (Col9a3) and Renin. Col9a3 expression was present at low levels in male and female gonads at 11.5 dpc. Thereafter, it was markedly up-regulated in the male, but remained very low in the female. Expression of Col9a3 was restricted to testicular cords and was also detected in testis of Kit(W/W-v) mice. Renin mRNA was first detected in testis at 12.5 dpc, increased thereafter, and reached a peak at 16.5 dpc. Renin mRNA was localized in cells of the interstitium and cells at the border between the gonad and mesonephros. Expression of Renin in the ovary was not detected using standard conditions.