Studying Neutrophil Function in vitro: Cell Models and Environmental Factors.
Studying Neutrophil Function in vitro: Cell Models and Environmental Factors.
复制标题
中性粒细胞功能的体外研究：细胞模型和环境因素。
DOI：
10.2147/jir.s284941
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发表时间：
2021
影响因子：
4.5
通讯作者：
Struyf S
中科院分区：
文献类型：
--
作者：
Blanter M;Gouwy M;Struyf S
中性粒细胞是血液中最丰富的免疫细胞类型，构成了抵御入侵病原体的第一道防线。尽管它们在许多疾病中起着重要作用，但由于它们的寿命短，无法在体外冷冻保存或扩增，因此研究它们具有挑战性。因此，对中性粒细胞的研究必须依赖于从人类供体的外周血中新鲜分离的细胞，从而在实验数据中引入供体依赖性变化。为了解决这些问题，研究人员试图开发适当的细胞模型，如细胞系。对于那些不能依赖于细胞模型的功能研究，中性粒细胞纯化和培养方案的标准化可能是一个解决方案。在这篇综述中，我们提供了最常用的中性粒细胞功能模型（HL-60，PLB-985，NB 4，Kasumi-1和诱导多能干细胞）的概述。此外，我们描述了葡萄糖浓度，pH值，氧张力和温度对中性粒细胞功能的影响。
Neutrophils are the most abundant immune cell type in the blood and constitute the first line of defense against invading pathogens. Despite their important role in many diseases, they are challenging to study due to their short life span and the inability to cryopreserve or expand them in vitro. Thus, research into neutrophils has to rely on cells freshly isolated from peripheral blood of human donors, introducing donor-dependent variation in the experimental data. To counteract these problems, researchers tried to develop adequate cell models, such as cell lines. For those functional studies that cannot rely on cell models, a standardization of protocols regarding neutrophil purification and culturing could be a solution. In this review, we provide an overview of the most commonly used models for neutrophil function (HL-60, PLB-985, NB4, Kasumi-1 and induced pluripotent stem cells). In addition, we describe the effects of glucose concentration, pH, oxygen tension and temperature on neutrophil function.