Synergistic interaction of interferon-beta and interferon-gamma in coxsackievirus B3-infected carrier cultures of human myocardial fibroblasts.
Synergistic interaction of interferon-beta and interferon-gamma in coxsackievirus B3-infected carrier cultures of human myocardial fibroblasts.
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发表时间：
1992
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通讯作者：
A. Heim;A. Canu;P. Kirschner;T. Simon;G. Mall;P. Hofschneider;R. Kandolf
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作者：
A. Heim;A. Canu;P. Kirschner;T. Simon;G. Mall;P. Hofschneider;R. Kandolf
在持续柯萨奇病毒 B3 感染的人心肌成纤维细胞载体培养物中，研究了人干扰素 β (IFN-β) 和人重组干扰素 γ (rIFN-γ) 的抗病毒作用，为期 21 天。通过定量原位杂交测定，低至 30 IU 的 IFN-β 加 10 IU rIFN-gamma/mL 的浓度可观察到协同作用，将平均病毒滴度从 6.0 x 10(7) pfu/mL 降低至 1.3 x 10(4) pfu/mL，并将感染细胞数从 14.4% 降低至 0.1%。较高浓度的干扰素（均>或= 30 IU/mL）与短暂的拮抗作用以及随后的抗病毒协同作用相关。使用 100 IU IFN-β 加 30 IU rIFN-gamma/mL，可持续消除感染性病毒，并在停止使用 IFN 后持续 6 周，而单一药物所需的浓度至少高出三倍。总之，我们的数据支持低剂量干扰素联合用药方案的概念，该方案可能有助于治疗肠道病毒性心脏病。
The antiviral effects of human interferon-beta (IFN-beta) and human recombinant interferon-gamma (rIFN-gamma) were studied in persistently coxsackievirus B3-infected carrier cultures of human myocardial fibroblasts over a period of 21 days. Synergism was observed with concentrations as low as 30 IU of IFN-beta plus 10 IU of rIFN-gamma/mL, reducing mean viral titers from 6.0 x 10(7) to 1.3 x 10(4) pfu/mL and number of infected cells from 14.4% to 0.1% as determined by quantitative in situ hybridization. Higher concentrations of IFNs (both > or = 30 IU/mL) were associated with transient antagonism followed by antiviral synergism. With 100 IU of IFN-beta plus 30 IU of rIFN-gamma/mL, elimination of infectious virus was consistently achieved and sustained for 6 weeks after cessation of IFN application, whereas at least threefold higher concentrations were required with single drugs. In summary, our data support a concept of low-dose IFN combination schedules that might become useful in the treatment of enteroviral heart disease.